School of Medicine for International Students, I-Shou University, Kaohsiung, Taiwan, R.O.C.
Department of Nursing, School of Medicine, I-Shou University, Kaohsiung, Taiwan, R.O.C.
Anticancer Res. 2020 Nov;40(11):6265-6271. doi: 10.21873/anticanres.14647.
BACKGROUND/AIM: Colorectal cancer (CRC) is one of the most common malignant tumors in the world. This study aimed to investigate the anticancer effect of the combination treatment of Ribociclib (LEE011) and 5-Fluorouracil (5-FU) on CRC cells.
HT-29 and SW480 cells were treated with LEE011, 5-FU, or the combination of LEE011 and 5-FU. Cell viability and cycle were investigated through 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay and flow cytometry. The expression of cell cycle-related proteins was determined through western blot.
The combined treatment of LEE011 with 5-FU synergistically reduced cell viability in HT-29 and SW480 cells. Specifically, it induced cell cycle arrest at the G phase, down-regulated the phosphorylation of retinoblastoma protein and the expression of p53.
LEE011 exhibited potential as an effective therapeutic inhibitor for the combination treatment of CRC patients.
背景/目的：结直肠癌（CRC）是世界上最常见的恶性肿瘤之一。本研究旨在探讨利匹韦林（LEE011）联合 5-氟尿嘧啶（5-FU）治疗结直肠癌细胞的抗癌作用。
采用 LEE011、5-FU 或 LEE011 和 5-FU 联合处理 HT-29 和 SW480 细胞。通过 3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺苯基)-2H-四唑比色法和流式细胞术检测细胞活力和细胞周期。通过 Western blot 检测细胞周期相关蛋白的表达。
LEE011 与 5-FU 联合治疗协同降低 HT-29 和 SW480 细胞的活力。具体而言，它诱导细胞周期在 G 期停滞，下调视网膜母细胞瘤蛋白的磷酸化和 p53 的表达。
LEE011 作为 CRC 患者联合治疗的有效治疗抑制剂具有潜力。
