Lamster I B, Mandella R D, Zove S M, Harper D S
Oral Health Research Center, Fairleigh S. Dickinson, Jr, College of Dental Medicine, Hackensack, NJ 07601.
Arch Oral Biol. 1987;32(5):329-33. doi: 10.1016/0003-9969(87)90087-2.
In human periodontal disease, there may be periods of exacerbation and remission. Definition of the homeostatic mechanisms in the periodontium may therefore be important in understanding the natural history of this disorder. The polyamines are biologically active amines involved in the regulation of cell growth, regeneration of tissue and modulation of inflammation. Their occurrence was examined in gingival crevicular fluid (GCF). Fifteen sites were evaluated in four patients with moderately advanced periodontitis before and after root planing and scaling, and 15 sites were evaluated in four patients with mild inflammatory gingivitis and no attachment loss. Polyamine analysis was by high-performance liquid chromatography. GCF from untreated sites in periodontitis patients contained the highest concentration of putrescine (10(4) greater than serum). This polyamine was detected in all periodontitis samples and 12 of 15 gingivitis samples. Significant differences were seen when the amount of putrescine/30 s sample was compared: periodontitis sites before treatment 1005.7 +/- 106.1 pmol; periodontitis sites after treatment 504.7 +/- 89.2 pmol; gingivitis sites 186.7 +/- 40.1 pmol. In contrast, spermidine and spermine were detected only occasionally. Thus putrescine may play an important homeostatic role in the periodontium.
在人类牙周病中，病情可能会有加重期和缓解期。因此，了解牙周组织中的稳态机制对于理解这种疾病的自然病程可能很重要。多胺是参与细胞生长调节、组织再生和炎症调节的生物活性胺。对龈沟液（GCF）中的多胺进行了检测。在4例中度晚期牙周炎患者进行根面平整和洁治前后，对15个位点进行了评估；在4例轻度炎症性牙龈炎且无附着丧失的患者中，对15个位点进行了评估。采用高效液相色谱法进行多胺分析。牙周炎患者未经治疗位点的龈沟液中腐胺浓度最高（比血清高10⁴倍）。在所有牙周炎样本和15个牙龈炎样本中的12个样本中均检测到了这种多胺。比较每30秒样本中腐胺的含量时发现了显著差异：治疗前牙周炎位点为1005.7±106.1 pmol；治疗后牙周炎位点为504.7±89.2 pmol；牙龈炎位点为186.7±40.1 pmol。相比之下，仅偶尔检测到亚精胺和精胺。因此，腐胺可能在牙周组织中发挥重要的稳态作用。
