First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Japan.
Department of Clinical and Laboratory, Faculty of Medicine, University of Yamanashi, Chuo, Japan.
Br J Cancer. 2021 Feb;124(3):570-573. doi: 10.1038/s41416-020-01134-7. Epub 2020 Oct 28.
In this study, we aimed to analyse human cancer cell-platelet interactions in functional cell analyses and explore the molecular mechanisms behind tumour progression. Various functional analyses of gastric cancer (GC) cells were performed after direct/indirect co-incubation with platelets derived from GC patients. Further detailed expression and signalling analyses were performed after co-culture with direct and indirect GC cells-platelet contact. Malignant behaviours of cancer cells, such as proliferation, migration, invasion and adhesion, were significantly enhanced after direct co-incubation with platelets. Microarray analyses demonstrated changes in multiple genes, including epithelial-mesenchymal transition (EMT)-related genes. Among them, matrix metalloproteinase 9 was notably upregulated, which was validated by quantitative reverse transcription-polymerase chain reaction and western blot. Further, this change was only observed after direct co-incubation with platelets. This study demonstrated that platelets from GC patients promote malignant behaviours of GC cells through EMT-related signalling, especially by direct contact with tumour cells.
在这项研究中，我们旨在通过功能细胞分析分析人类癌细胞-血小板相互作用，并探索肿瘤进展背后的分子机制。在与源自胃癌患者的血小板直接/间接共孵育后，对胃癌 (GC) 细胞进行了各种功能分析。在与直接和间接 GC 细胞-血小板接触共培养后，进行了更详细的表达和信号转导分析。癌细胞的恶性行为，如增殖、迁移、侵袭和黏附，在与血小板直接共孵育后显著增强。微阵列分析显示多个基因发生变化，包括上皮-间充质转化 (EMT) 相关基因。其中，基质金属蛋白酶 9明显上调，这通过定量逆转录聚合酶链反应和 Western blot 得到验证。此外，这种变化仅在与血小板直接共孵育后观察到。本研究表明，来自胃癌患者的血小板通过 EMT 相关信号通路促进胃癌细胞的恶性行为，特别是通过与肿瘤细胞的直接接触。
