Spremulli L, Kraus B L
Department of Chemistry, University of North Carolina, Chapel Hill 27514.
Biochem Biophys Res Commun. 1987 Sep 30;147(3):1077-81. doi: 10.1016/s0006-291x(87)80180-8.
The effects of cations and ribosome dissociation factors on the equilibrium between the bovine mitochondrial ribosome and its subunits has been investigated. As observed with other ribosomes, Mg2+ ions promote subunit association while monovalent cations promote subunit dissociation. E. coli IF-3 will prevent the reassociation of mitochondrial 28 S and 39 S subunits. However, at least 5-fold higher concentrations of IF-3 are required with mitochondrial subunits than are required with bacterial subunits. The cytoplasmic factor eIF-6, has no detectable activity in preventing mitochondrial ribosomal subunit association.
已对阳离子和核糖体解离因子对牛线粒体核糖体及其亚基之间平衡的影响进行了研究。正如在其他核糖体中观察到的那样，Mg2+离子促进亚基缔合，而单价阳离子促进亚基解离。大肠杆菌IF-3可阻止线粒体28 S和39 S亚基的重新缔合。然而，线粒体亚基所需的IF-3浓度比细菌亚基所需的浓度至少高5倍。细胞质因子eIF-6在阻止线粒体核糖体亚基缔合方面没有可检测到的活性。
