Safarík I
Laboratory of Analytical Chemistry, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
Biotechnol Appl Biochem. 1987 Aug;9(4):323-4.
An insoluble chromogenic substrate for the determination of proteolytic activity was prepared by heating azocasein in a thin layer at 200 degrees C for 4 h in a hot-air thermostat. The activity of an extracellular bacterial proteinase produced by Bacillus sp. was determined with this new substrate.
