使用α-平滑肌肌动蛋白（α-SMA）和波形蛋白对正常口腔黏膜、口腔上皮发育异常及口腔鳞状细胞癌中肌成纤维细胞表达的比较：一项免疫组织化学研究
Comparison of expression of myofibroblasts in normal oral mucosa, oral epithelial dysplasia, and oral squamous cell carcinoma using α-SMA and vimentin: An immunohistochemical study.
作者信息
Mahajan Amani, Manhas Andleeb, Aneja Tania, Parhar Swati, Kumari Priyanka, Singh Harkanwal P
机构信息
Department of Oral Pathology and Microbiology, Swami Devi Dyal Hospital and Dental College, Golpura, Barwala, Panchkula, Haryana, India.
Department of Medical, Health and Family Welfare, Uttarakhand, India.
出版信息
J Family Med Prim Care. 2020 Aug 25;9(8):4368-4374. doi: 10.4103/jfmpc.jfmpc_172_20. eCollection 2020 Aug.
BACKGROUND
Myofibroblasts are thought to play critical roles in inflammation, growth, repair, premalignancy, and malignancy. This study was done to evaluate, compare and co- relate the progressive increase in the immunohistochemical expression of myofibroblasts in normal oral mucosa, epithelial dysplasia, and oral squamous cell carcinoma (OSCC). To compare and co-relate the expression of myofibroblasts in normal oral mucosa, epithelial dysplasia, and oral squamous cell carcinoma. To co-relate the progressive increase in myofibroblasts expression in normal oral mucosa, epithelial dysplasia, and oral squamous cell carcinoma.
MATERIALS AND METHOD
Forty-nine paraffin-embedded tissue blocks with 7 cases of normal oral mucosa, 21 cases of epithelial dysplasia, and 21 diagnosed cases of OSCCs were studied. The samples were subjected to heat-induced antigen retrieval methods followed by staining using primary mouse monoclonal antibodies against α-smooth muscle actin (SMA) and vimentin. Staining index of all the sections was calculated. Statistical analysis was performed using the Kruskal-Wallis test, Mann-Whitney U test, and Chi-square test. Values of less than or equal to 0.05 ( ≤ 0.05) were considered statistically significant.
RESULTS
Statistically significant staining index was obtained by α-SMA and vimentin between normal oral mucosa, epithelial dysplasia, and OSCC.
CONCLUSION
Myofibroblast may play a role only during initial tumorigenesis that is the conversion of severe dysplasia into OSCC.
背景
肌成纤维细胞被认为在炎症、生长、修复、癌前病变和恶性肿瘤中起关键作用。本研究旨在评估、比较和关联正常口腔黏膜、上皮发育异常和口腔鳞状细胞癌（OSCC）中肌成纤维细胞免疫组化表达的逐渐增加情况。比较和关联正常口腔黏膜、上皮发育异常和口腔鳞状细胞癌中肌成纤维细胞的表达。关联正常口腔黏膜、上皮发育异常和口腔鳞状细胞癌中肌成纤维细胞表达的逐渐增加情况。
材料与方法
研究了49个石蜡包埋组织块，其中包括7例正常口腔黏膜、21例上皮发育异常和21例确诊的口腔鳞状细胞癌病例。样本采用热诱导抗原修复方法，然后用抗α平滑肌肌动蛋白（SMA）和波形蛋白的小鼠单克隆一抗进行染色。计算所有切片的染色指数。使用Kruskal-Wallis检验、Mann-Whitney U检验和卡方检验进行统计分析。小于或等于0.05（≤0.05）的值被认为具有统计学意义。
结果
α-SMA和波形蛋白在正常口腔黏膜、上皮发育异常和口腔鳞状细胞癌之间获得了具有统计学意义的染色指数。
结论
肌成纤维细胞可能仅在初始肿瘤发生过程中起作用，即重度发育异常转化为口腔鳞状细胞癌的过程。
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