McKenzie M L, Thomaskutty K G, Swift M L, Pointer R H
Department of Biochemistry, Howard University College of Medicine, Washington, DC 20059.
Biochem Med Metab Biol. 1987 Aug;38(1):81-7. doi: 10.1016/0885-4505(87)90065-x.
Adipose tissue segments excised from normal rats and from rats rendered experimentally hypothyroid and hyperthyroid retained insulin responsiveness when studied in vitro. Basal rates of glucose oxidation to CO2, conversion into glyceride-glycerol, fatty acids, and total lipids, and the activities of pyruvate dehydrogenase and fatty acid synthetase were enhanced in fat pads from hyperthyroid rats when compared with values seen with tissue from euthyroid animals. The response of each of these parameters was further enhanced by treating tissue from hyperthyroid rats in vitro with insulin. Basal rates of glucose oxidation and the activities of pyruvate dehydrogenase and fatty acid synthetase were depressed as a result of hypothyroidism. However, all of these values could be restored to levels approaching the values seen in the basal state for euthyroid rats when tissue segments from hypothyroid rats were incubated in vitro with insulin. The basal rates for glucose conversion into glyceride-glycerol, fatty acids, and total lipids were not changed by hypothyroidism but retained insulin responsiveness. These data suggest that the insulin-effector system in adipose tissue is not altered by thyroid status and that thyroid hormones may act independently with insulin to regulate glucose and lipid metabolism in this tissue at multiple intracellular metabolic sites.
从正常大鼠以及实验性甲状腺功能减退和甲状腺功能亢进大鼠身上切除的脂肪组织片段，在体外研究时保留了胰岛素反应性。与甲状腺功能正常动物的组织相比，甲状腺功能亢进大鼠脂肪垫中葡萄糖氧化为二氧化碳的基础速率、转化为甘油 - 甘油、脂肪酸和总脂质的速率，以及丙酮酸脱氢酶和脂肪酸合成酶的活性均有所增强。用胰岛素体外处理甲状腺功能亢进大鼠的组织，这些参数中的每一个的反应都会进一步增强。甲状腺功能减退导致葡萄糖氧化的基础速率以及丙酮酸脱氢酶和脂肪酸合成酶的活性降低。然而，当甲状腺功能减退大鼠的组织片段在体外与胰岛素一起孵育时，所有这些值都可以恢复到接近甲状腺功能正常大鼠基础状态下的值。甲状腺功能减退并未改变葡萄糖转化为甘油 - 甘油、脂肪酸和总脂质的基础速率，但保留了胰岛素反应性。这些数据表明，脂肪组织中的胰岛素效应系统不受甲状腺状态的影响，并且甲状腺激素可能与胰岛素独立作用，在该组织的多个细胞内代谢位点调节葡萄糖和脂质代谢。
