Ji Min Gun, Lee Yeong Ro, Nam Youn Hee, Castañeda Rodrigo, Hong Bin Na, Kang Tong Ho
Department of Oriental Medicine Biotechnology, College of Life Sciences and Graduate School of Biotechnology, Kyung Hee University, Global Campus, Gyeonggi-do 17104, Republic of Korea.
ACS Omega. 2020 Sep 22;5(41):26374-26381. doi: 10.1021/acsomega.0c02472. eCollection 2020 Oct 20.
Immunostimulatory activity comprises specific and nonspecific immune responses stimulated by internal and external factors. Arabinoxylan is well known for its immunostimulatory activity and , although the biological activities of arabinoxylan oligosaccharides depend on their structural features. In this study, we aimed to evaluate and the immunostimulatory activity of high-content active arabinoxylan (HCAA) obtained from rice bran through bioconversion by microorganisms and acid hydrolysis. Three microorganisms, , , and , and three different acid concentrations of hydrochloric acid (5, 10, and 20%) and acetic acid (25, 50, and 75%) were used for producing HCAA. HPLC analysis of arabinose and xylose content revealed that fermentation with followed by hydrolysis with 5% hydrochloric acid was the most efficient to produce HCAA. GPC analysis of HCAA indicates that HCAA is a complex of various forms of saccharides and shows an average molecular weight of 625. Further, evaluation disclosed that exposure to HCAA (10-200 μg/mL) increased cell viability in mice splenic cells and RAW 264.7 cells. Additionally, exposure of mice to oral administration of HCAA (100 mg/kg) for 4-7 days increased lymphokine-activated killer (LAK)- and macrophage-mediated cytotoxic activity in cancer cells (YAC-1). Furthermore, exposure to HCAA and oral administrations in mice revealed increased interferon-γ (IFN-γ) and interleukin-10 (IL-10) protein expression through western blot analysis in RAW 264.7 cells and isolated splenic cells. Our results suggest that HCAA developed by bioconversion and acid hydrolysis may enhance immune responses and .
免疫刺激活性包括由内部和外部因素刺激产生的特异性和非特异性免疫反应。阿拉伯木聚糖以其免疫刺激活性而闻名，尽管阿拉伯木聚糖寡糖的生物活性取决于其结构特征。在本研究中，我们旨在评估通过微生物生物转化和酸水解从米糠中获得的高含量活性阿拉伯木聚糖（HCAA）的免疫刺激活性。使用三种微生物，即[具体微生物名称1]、[具体微生物名称2]和[具体微生物名称3]，以及三种不同酸浓度的盐酸（5%、10%和20%）和乙酸（25%、50%和75%）来生产HCAA。对阿拉伯糖和木糖含量的HPLC分析表明，先用[具体微生物名称1]发酵，然后用5%盐酸水解是生产HCAA最有效的方法。对HCAA的GPC分析表明，HCAA是多种糖类形式的复合物，平均分子量为625。此外，评估发现，暴露于HCAA（10 - 200μg/mL）可提高小鼠脾细胞和RAW 264.7细胞的细胞活力。此外，给小鼠口服HCAA（100mg/kg）4 - 7天，可增加癌细胞（YAC - 1）中淋巴因子激活的杀伤细胞（LAK）和巨噬细胞介导的细胞毒性活性。此外，对小鼠进行HCAA暴露和口服给药后，通过蛋白质印迹分析发现RAW 264.7细胞和分离的脾细胞中干扰素 - γ（IFN - γ）和白细胞介素 - 10（IL - 10）蛋白表达增加。我们的结果表明，通过生物转化和酸水解开发的HCAA可能增强免疫反应。
