人诱导多能干细胞向肺神经内分泌细胞分化的方案。
Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells.
机构信息
Hastings Center for Pulmonary Research and Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
出版信息
STAR Protoc. 2020 Jul 24;1(2):100068. doi: 10.1016/j.xpro.2020.100068. eCollection 2020 Sep 18.
Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to study lung diseases characterized by PNEC dysfunction. For complete details on the use and execution of this protocol, please refer to Hor et al. (2020).
肺神经内分泌细胞（PNECs）是肺气道上皮中的感觉细胞。在这里，我们提供了一个从人诱导多能干细胞（iPSCs）产生诱导性 PNECs（iPNECs）的详细方案。iPNECs 的细胞和分子特征与原代人 PNECs 相似。原代人 PNECs 非常罕见，仅占成人肺的 1%。因此，患者特异性 iPNECs 的自我更新来源有助于创建可重复的人类细胞模型，以研究以 PNEC 功能障碍为特征的肺部疾病。有关该方案使用和执行的完整详细信息，请参阅 Hor 等人。（2020）。
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