在生理切变应力下对人诱导多能干细胞源性血管内皮细胞的分化和鉴定。
Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.
机构信息
Department of Biomedical Engineering, Duke University, Durham, NC 27713, USA.
出版信息
STAR Protoc. 2021 Mar 17;2(2):100394. doi: 10.1016/j.xpro.2021.100394. eCollection 2021 Jun 18.
Abstract
Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).
摘要
诱导多能干细胞 (iPSCs) 为生成内皮细胞 (ECs) 提供了一个潜在的无限来源，可用于众多应用。在这里，我们描述了一种 7 天的方案，使用小分子调节特定转录因子从 350 万个人 iPSCs 中分化出多达 5500 万个血管内皮细胞 (viECs)。我们还描述了一个平行板流动腔系统，用于在生理剪切应力下研究 EC 的行为。有关该方案使用和执行的完整详细信息，请参阅 Atchison 等人。（2020 年）。
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