Viña-Almunia Jose, Pellicer-Chover Hilario, García-Mira Berta, Romero-Millán Javier, Peñarrocha-Oltra David, Peñarrocha-Diago Miguel
Oral Surgery and Implantology Unit, Department of Stomatology, Faculty of Medicine and Dentistry, University of Valencia, Gascó Oliag 1, 46010, Valencia, Spain.
Int J Implant Dent. 2020 Oct 28;6(1):71. doi: 10.1186/s40729-020-00262-2.
The objective of the study was to evaluate the relation between occlusal loading and peri-implant crevicular fluid cytokine expression in patients with implant-supported complete fixed prostheses in both arches.
A prospective longitudinal clinical study was performed at a university clinic. Fifteen patients were selected and 11 were included. All patients had bimaxillary implant-supported complete fixed ceramo-metallic prostheses loaded at least 12 months before the beginning of the study. Allocation was established for each patient using a computerized occlusal analysis system. The test implant was the maxillary implant closest to the point of highest occlusal loading. The maxillary implant with least loading was the control implant. Occlusal adjustment was performed using a round diamond burr. This occlusal distribution was verified with the occlusal analysis system. Expression of cytokines from peri-implant crevicular fluid (TNF-α, IL-10, IL-6, IL-1β, IL-8) were recorded and analyzed in both test and control implants before (baseline: T0) and 2 (T1) and 12 months (T2) after occlusal adjustment. The Brunner-Langer non-parametric test was performed.
At T0, the expression of IL-10 was significantly higher in the test group implants (p = 0.018). Between T0 and T1, the expression of all the cytokines decreased in the implants of both groups with statistically significant differences, except for TNF (p = 0.271). When comparing both groups at T1, there was no statistically significant difference in any of the analyzed cytokines. At T2, TNF-α suffered when compared with baseline, a statistical decrease in both study and control implants (p < 0,001). At T2, there were no statistically significant differences between groups in any of the cytokines analyzed.
Implants with higher occlusal load presented higher expression of IL-10 in peri-implant crevicular fluid. Occlusal adjustment produced a decrease in the expression of all the analyzed cytokines, both in test and control implants.
本研究的目的是评估双侧种植支持式全口固定义齿患者的咬合负荷与种植体周围龈沟液细胞因子表达之间的关系。
在一所大学诊所进行了一项前瞻性纵向临床研究。选择了15名患者，纳入11名。所有患者在研究开始前至少12个月佩戴了双侧种植支持式全口金属烤瓷固定义齿。使用计算机化咬合分析系统为每位患者确定分配方案。测试种植体是上颌最接近咬合负荷最高点的种植体。上颌负荷最小的种植体为对照种植体。使用圆形金刚石车针进行咬合调整。用咬合分析系统验证这种咬合分布。在咬合调整前（基线：T0）、调整后2个月（T1）和12个月（T2）记录并分析测试种植体和对照种植体的种植体周围龈沟液中细胞因子（TNF-α、IL-10、IL-6、IL-1β、IL-8）的表达。进行Brunner-Langer非参数检验。
在T0时，测试组种植体中IL-10的表达显著更高（p = 0.018）。在T0和T1之间，两组种植体中所有细胞因子的表达均下降，除TNF外（p = 0.271），差异具有统计学意义。在T1时比较两组，所分析的任何细胞因子均无统计学显著差异。在T2时，与基线相比，TNF-α在研究种植体和对照种植体中均出现统计学下降（p < 0.001）。在T2时，所分析的任何细胞因子在两组之间均无统计学显著差异。
咬合负荷较高的种植体在种植体周围龈沟液中IL-10的表达较高。咬合调整使测试种植体和对照种植体中所有分析的细胞因子的表达均下降。
Zotero 插件