Department of Pathology, Gazi Yaşargil Education and Research Hospital, Diyarbakır, Turkey.
Department of Gastroenterology, Dicle University, Faculty of Medicine, Diyarbakır, Turkey.
Pol J Pathol. 2020;71(3):221-228. doi: 10.5114/pjp.2020.99788.
Although many studies have been conducted to explore the relationship between mast cells (MC) and angiogenesis, comparison of this relationship with tumor necrosis has not been investigated to the best of our knowledge. Therefore, the relationship between MC and neovascularization in stomach, lung and ovarian malignant epithelial tumors (165 cases) in necrotizing or non-necrotizing cases was explored in this study. We immunohistochemically studied anti-mast cell tryptase antibody for MC and anti-CD34 antibody for vascular structures. MCs in the intra- tumoral and peritumoral fields, as well as vascular structures with luminal and monocellular appearances in the intratumoral field, were counted in each sample. Ten magnification fields were analyzed for each sample. In stomach and lung cases, the non-necrotizing group exhibited a greater number of MC and vascular structures in total. In ovarian cases, more MCs were counted overall in the necrotizing group, but there were fewer vascular structures. The increase in the number of MC and vascular structures in lungs and stomach in the non-necrotizing group supports the theory that MCs are involved in tumor progression. Necrosis, which can be induced on the basis of restricted neovascularization through inhibition of MCs in lung and stomach tumors, may be a treatment method.
尽管已经进行了许多研究来探讨肥大细胞（MC）与血管生成之间的关系，但据我们所知，尚未对其与肿瘤坏死之间的关系进行比较。因此，本研究旨在探讨胃、肺和卵巢恶性上皮肿瘤（165 例）中坏死或非坏死病例中 MC 与新生血管之间的关系。我们通过免疫组织化学研究了抗 MC 类胰蛋白酶抗体和抗 CD34 抗体来检测 MC 和血管结构。在每个样本中，我们对肿瘤内和肿瘤周围区域的 MC 以及肿瘤内管腔和单细胞外观的血管结构进行了计数。每个样本分析了 10 个放大视野。在胃和肺病例中，非坏死组的 MC 和血管结构总数更多。在卵巢病例中，坏死组的 MC 计数总体上更多，但血管结构更少。非坏死组中肺和胃中 MC 和血管结构数量的增加支持了 MC 参与肿瘤进展的理论。在肺和胃肿瘤中，通过抑制 MC 来限制血管生成，从而导致的坏死可能是一种治疗方法。
