Lacasse J, Kreft C, Gutkowska J, Genest J, Cantin M
Multidisciplinary Research Group on Hypertension, Clinical Research Institute of Montreal, Que., Canada.
Can J Physiol Pharmacol. 1987 Jul;65(7):1409-15. doi: 10.1139/y87-221.
Trypsin- or collagenase-dispersed renal cortical cells from newborn mice were filtered and cultured. The cultures comprised 25% juxtaglomerular cells as identified by immunocytochemistry. Renin was measured by radioimmunoassay in the culture medium and in the cells at various time intervals. This in vitro system was responsive to isoproterenol, which stimulated renin release in a dose-dependent manner.
对新生小鼠经胰蛋白酶或胶原酶分散处理的肾皮质细胞进行过滤并培养。通过免疫细胞化学鉴定，培养物中包含25%的球旁细胞。在不同时间间隔，通过放射免疫分析法测定培养基和细胞中的肾素。该体外系统对异丙肾上腺素敏感，异丙肾上腺素能以剂量依赖的方式刺激肾素释放。
