Department of Surgery, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan, Chungcheongnam-do 31151, Korea.
Soonchunhyang Medical Science Research Institute, College of Medicine, Soonchunhyang University 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan, Chungcheongnam-do 330-722, Korea.
Int J Mol Sci. 2020 Oct 26;21(21):7928. doi: 10.3390/ijms21217928.
HJURP is a key factor for CENP-A deposition and maintenance in centromeres. The role of mis-regulation of histone chaperones in cancer initiation and progression has been studied. However, its role in colorectal cancer is still unclear. In this study, we aimed to evaluate the expression of HJURP in 162 colorectal cancer tissue. To investigate the function of HJURP in the colorectal cancer cell, we suppressed HJURP expression by siRNA and confirmed proliferation, migration, invasion, and anchorage independent of colony forming ability. The association between HJURP expression levels and clinicopathological factors was evaluated in 162 CRC tissues using immunohistochemistry. The overall survival rate in patients of HJURP high expression was higher than those in HJURP low expression in CRC. Suppressing HJURP expression decreased cellular proliferation, invasion, and migration in four CRC cell lines: HT29, HCT116, SW480, SW620 in vitro study. Our findings revealed that the knockdown of HJURP suppressed the proliferation, migration, invasion, and tumorigenicity in CRC cells. Due to its strong association with CRC, HJURP could be a potential prognostic biomarker and a novel target for drug discovery.
HJURP 是 CENP-A 在着丝粒中沉积和维持的关键因素。组蛋白伴侣的失调在癌症的发生和发展中的作用已经被研究过。然而，其在结直肠癌中的作用仍不清楚。在本研究中，我们旨在评估 162 例结直肠癌组织中 HJURP 的表达。为了研究 HJURP 在结直肠癌细胞中的功能，我们通过 siRNA 抑制 HJURP 的表达，并证实了增殖、迁移、侵袭和锚定非依赖性集落形成能力。免疫组织化学法评估了 162 例 CRC 组织中 HJURP 表达水平与临床病理因素的关系。在 CRC 中，HJURP 高表达患者的总生存率高于 HJURP 低表达患者。体外研究表明，抑制 HJURP 表达可降低 HT29、HCT116、SW480 和 SW620 四种 CRC 细胞系的细胞增殖、侵袭和迁移。我们的研究结果表明，HJURP 的敲低抑制了 CRC 细胞的增殖、迁移、侵袭和致瘤性。由于 HJURP 与 CRC 有很强的相关性，因此它可能是一个有潜力的预后生物标志物和药物发现的新靶点。
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