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枸杞根茎、茎和叶中的维生素C类似物2-O-β-D-吡喃葡萄糖基-L-抗坏血酸

The Vitamin C Analogue 2-O-β-D-Glucopyranosyl-L-ascorbic Acid in Rhizomes, Stems and Leaves of Lycium barbarum.

作者信息

Bubloz Carole, Udrisard Isabelle, Micaux Fabrice, Piantini Umberto, Amini-Rentsch Lara, Marti Roger, Andlauer Wilfried

机构信息

HES-SO Valais-Wallis, Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland, Route du Rawyl 47, CH-1950 Sion, Switzerland; BFH-HAFL, Bern University of Applied Sciences, Länggasse 85, CH-3052 Zollikofen, Switzerland.

HES-SO Valais-Wallis, Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland, Route du Rawyl 47, CH-1950 Sion, Switzerland.

出版信息

Chimia (Aarau). 2020 Oct 28;74(10):828-830. doi: 10.2533/chimia.2020.828.

DOI:10.2533/chimia.2020.828
PMID:33115572
Abstract

Awareness of health benefits of goji berries coming from their bioactive compounds, mostly antioxidants like ascorbic acid, has grown. Recently, an ascorbic acid analogue from goji berries, the 2-O-β-D-glucopyranosyl-L-ascorbic acid has been reported. In rats, the analogue is absorbed intact and in the form of free vitamin C and consequently has been proposed as a provitamin C. Synthesis of the analogue is demanding and laborious and therefore reliable natural sources are searched. Knowledge concerning the analogue's occurrence in other parts of goji plant is lacking. The aim of this study was to evaluate the contents of 2-O-β-D-glucopyranosyl-L-ascorbic acid in rhizomes, stems and leaves from . Rhizomes, stems and leaveswere extracted and the content of 2-O-β-D-glucopyranosyl-L-ascorbic acid and non glucosylated, free ascorbic acid was determined by HPLC-DAD. 2-O-β-D-glucopyranosyl-L-ascorbic acid was found in all goji plant tissues investigated. Based on dry weight, 3.34 mg/100 g were found in the leaves, 4.05 mg/100 g in the stems and up to 12.6 mg/100 g in the rhizomes. Nevertheless, the analogue content in goji berries is much higher (40 to 280 mg/100 g dry weight). The present study confirmed the presence of 2-O-β-D-glucopyranosyl-L-ascorbic acid in rhizomes, stems and leaves of . However, their content compared to goji berries is considerably lower.

摘要

人们越来越意识到枸杞因其生物活性化合物(主要是抗坏血酸等抗氧化剂)而对健康有益。最近,已报道了一种来自枸杞的抗坏血酸类似物,即2-O-β-D-吡喃葡萄糖基-L-抗坏血酸。在大鼠中,该类似物以游离维生素C的形式完整吸收,因此被提议作为维生素C原。该类似物的合成要求高且费力,因此正在寻找可靠的天然来源。目前缺乏关于该类似物在枸杞植株其他部位存在情况的知识。本研究的目的是评估枸杞根茎、茎和叶中2-O-β-D-吡喃葡萄糖基-L-抗坏血酸的含量。对根茎、茎和叶进行了提取,并通过高效液相色谱-二极管阵列检测法测定了2-O-β-D-吡喃葡萄糖基-L-抗坏血酸和非糖基化的游离抗坏血酸的含量。在所研究的枸杞植株所有组织中均发现了2-O-β-D-吡喃葡萄糖基-L-抗坏血酸。以干重计,叶中含量为3.34毫克/100克,茎中为4.05毫克/100克,根茎中高达12.6毫克/100克。然而,枸杞果实中该类似物的含量要高得多(干重为40至280毫克/100克)。本研究证实了枸杞根茎、茎和叶中存在2-O-β-D-吡喃葡萄糖基-L-抗坏血酸。然而,与枸杞果实相比,它们的含量要低得多。

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