Yue B Y, Elvart J L
University of Illinois, Department of Ophthalmology, Lions of Illinois Eye Research Institute, Chicago 60612.
Curr Eye Res. 1987 Aug;6(8):959-67. doi: 10.3109/02713688709034867.
We compared the incorporation of radioactive precursors into glycosaminoglycans by cynomolgus monkey trabecular meshwork cells in tissue culture and in organ culture. Hyaluronic acid and a variety of sulfated glycosaminoglycans were synthesized in both systems. In organ cultures, the ratio of chondroitin 6- to chondroitin 4-sulfate was higher than that found in tissue cultures. Also, a greater proportion of total glycosaminoglycans found in the organ culture medium was represented by hyaluronic acid. The higher production of chondroitin 6-sulfate and hyaluronic acid, as noted in some embryonic systems, suggested that cells in organ cultures may resemble an acute wound healing or an early developmental state more closely than did cells in tissue cultures.
我们比较了食蟹猴小梁网细胞在组织培养和器官培养中放射性前体掺入糖胺聚糖的情况。在这两种培养系统中均合成了透明质酸和多种硫酸化糖胺聚糖。在器官培养中，硫酸软骨素6-硫酸酯与硫酸软骨素4-硫酸酯的比例高于组织培养中的比例。此外，器官培养基中发现的总糖胺聚糖中，透明质酸所占比例更大。如在一些胚胎系统中所观察到的，硫酸软骨素6-硫酸酯和透明质酸的产量较高，这表明器官培养中的细胞可能比组织培养中的细胞更类似于急性伤口愈合或早期发育状态。
