Klimes I, Zórad S, Svábová E, Macho L
Center of Physiological Sciences, Slovak Academy of Sciences, Bratislava, Czehoslovakia.
Endocrinol Exp. 1987 Sep;21(3):209-17.
Effect of inhalation of an air-n-hexane mixture on insulin degradation in rat blood in vitro was studied under simultaneous monitoring of n-hexane concentrations in blood (using gas chromatography) and in the inhaled air (using infrared analysis). Inhalation of n-hexane vapours in a concentration of 54,694 +/- 1933 ppm (197 +/- 7 g m-3) resulted in blood n-hexane saturation within 15 min. (0.18 +/- 0.023 mg ml-1) associated with a statistically increased (P less than 0.002) insulin degradation in the blood of exposed rats as compared to control animals 0.152 +/- 0.03 vs. 0.053 +/- 0.003). To rule out possible effects of stress on the results obtained, effects of both, 20 min restraint stress (RS) and equally long handling (HAN) on insulin degradation were also estimated. The data thus obtained were compared with insulin degradation in control (C) rats which were killed by decapitation in the animal room. No significant differences were observed between the individual groups (C: 0.055 +/- 0.08; RS: 0.068 +/- 0.01; HAN: 0.069 +/- 0.015). In vitro addition of n-hexane to pure plasma or to a mixture of blood elements revealed that degradation activities present in the cellular blood compartment are required for the n-hexane-induced enhancement of insulin degradation in the blood to occur.
在体外同时监测血液中己烷浓度（使用气相色谱法）和吸入空气中己烷浓度（使用红外分析法）的情况下，研究了吸入空气 - 正己烷混合物对大鼠血液中胰岛素降解的影响。吸入浓度为54,694±1933 ppm（197±7 g m-3）的正己烷蒸气会导致血液在15分钟内达到己烷饱和（0.18±0.023 mg ml-1）。与对照动物相比，暴露大鼠血液中的胰岛素降解在统计学上显著增加（P＜0.002）（0.152±0.03对0.053±0.003）。为了排除应激对所得结果的可能影响，还评估了20分钟束缚应激（RS）和同样时长的处理（HAN）对胰岛素降解的影响。将由此获得的数据与在动物房通过断头处死的对照（C）大鼠的胰岛素降解情况进行比较。各实验组之间未观察到显著差异（C组：0.055±0.08；RS组：0.068±0.01；HAN组：0.069±0.015）。在体外向纯血浆或血液成分混合物中添加正己烷表明，血液细胞成分中存在的降解活性是正己烷诱导血液中胰岛素降解增强所必需的。
