Yang Xing, Luo Yuan, Li Sanpeng, Xu Xiuli, Bao Yingxia, Yang Jiaming, Ouyang Defang, Fan Xingxing, Gong Ping, Cai Lintao
Guangdong Key Laboratory of Nanomedicine, CAS-HK Joint Lab for Biomaterials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Bioeng Biotechnol. 2020 Oct 2;8:903. doi: 10.3389/fbioe.2020.00903. eCollection 2020.
A simple and facile one-step method was developed to construct a small molecular prodrug amphiphile self-assembled organic dots CPPG with aggregation-induced emission (AIE) characteristics. Diphenylalanine peptide (FF), which is the essential moiety of the self-assembling peptide-drug conjugate and as its core recognition motifs for molecular self-assembly. In addition, the D-glucose transported protein (GLUT), which is one of the important nutrient transporters and is overexpressed in cancer cells. The conjugation of glycosyl further endues the nanoparticle with good biocompatibility and tumor-targeting ability. Taking advantages of both the cancer cell-targeting capability of small molecular prodrug amphiphile CPPG and the AIE aggregates with strong emission, the prepared CPPG AIE dots can target cancer cells specifically and inhibit the proliferation of cancer cells with good biocompatibility and photostability. Based on the general approach, types of universal organic fluorescent nanoprobes could be facilely constructed for imaging applications and biological therapeutics, which possess the properties of specific recognition and high brightness.
开发了一种简单易行的一步法来构建具有聚集诱导发光（AIE）特性的小分子前药两亲性自组装有机点CPPG。二苯基丙氨酸肽（FF）是自组装肽-药物缀合物的基本部分，作为其分子自组装的核心识别基序。此外，D-葡萄糖转运蛋白（GLUT）是重要的营养转运蛋白之一，在癌细胞中过表达。糖基的缀合进一步赋予纳米颗粒良好的生物相容性和肿瘤靶向能力。利用小分子前药两亲性CPPG的癌细胞靶向能力和具有强发射的AIE聚集体，制备的CPPG AIE点可以特异性靶向癌细胞，并以良好的生物相容性和光稳定性抑制癌细胞的增殖。基于这种通用方法，可以轻松构建各种通用的有机荧光纳米探针用于成像应用和生物治疗，这些探针具有特异性识别和高亮度的特性。
