Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:310-317. doi: 10.1016/j.msec.2018.09.035. Epub 2018 Sep 12.
Amphiphilic copolymers play a paramount role in the fabrication of fluorescent polymeric nanoparticles (FPNs) through the self-assembly procedure. In this work, novel hydrazine hydrate cross-linked amphiphilic poly(PEG‑co‑FHMA) copolymers were constructed via reversible addition-fragmentation chain transfer (RAFT) polymerization, containing an aggregation-induced emission (AIE) active hydrophobic moiety and a hydrophilic poly(ethylene glycol) (PEG) group. Different characterization techniques have been employed to confirm their successful synthesis. Due to their amphiphilic property, the resulting poly(PEG‑co‑FHMA) copolymers can self-assemble into FPNs in aqueous solution and form poly(PEG‑co‑FHMA) FPNs with size ranging from 100 to 200 nm. The investigation of photophysical properties demonstrated poly(PEG‑co‑FHMA) FPNs possess strong fluorescence, large Stokes shift, excellent AIE characteristic, low critical micelle concentration and remarkable photostability. Biological assay results suggested that these cross-linked AIE-active FPNs are of low toxicity and excellent cell dyeing performances. All of these features make them promising candidates for biomedical applications. As compared with typical AIE-active FPNs based on the synthetic AIE-active compounds, the novel cross-linked AIE-active FPNs based on the Schiff base is rather simple, good designable and universal. More importantly, this strategy could also be adopted for preparation of a large number of AIE-active FPNs because of the well designability of copolymers and salicylaldehyde derivatives. Thus this work will provide a novel route for preparation of multifunctional AIE-active FPNs in a rather facile manner.
两亲性共聚物在通过自组装程序制备荧光聚合物纳米粒子（FPN）方面起着至关重要的作用。在这项工作中，通过可逆加成-断裂链转移（RAFT）聚合构建了新型水合肼交联的两亲性聚（PEG-co-FHMA）共聚物，其含有聚集诱导发射（AIE）活性疏水性部分和亲水性聚（乙二醇）（PEG）基团。不同的表征技术已被用于确认其成功合成。由于其两亲性，所得的聚（PEG-co-FHMA）共聚物可以在水溶液中自组装成 FPN，并形成尺寸在 100 至 200nm 之间的聚（PEG-co-FHMA）FPN。光物理性质的研究表明，聚（PEG-co-FHMA）FPN 具有强荧光、大斯托克斯位移、优异的 AIE 特性、低临界胶束浓度和显著的光稳定性。生物测定结果表明，这些交联的 AIE 活性 FPN 具有低毒性和优异的细胞染色性能。所有这些特性使它们成为生物医学应用的有前途的候选物。与基于合成 AIE 活性化合物的典型 AIE 活性 FPN 相比，基于席夫碱的新型交联 AIE 活性 FPN 更为简单、设计性好且通用性强。更重要的是，由于共聚物和水杨醛衍生物的良好设计性，这种策略还可以用于制备大量的 AIE 活性 FPN。因此，这项工作将为以相当简单的方式制备多功能 AIE 活性 FPN 提供一条新途径。
