Majka Zuzanna, Czamara Krzysztof, Wegrzyn Piotr, Litwinowicz Radoslaw, Janus Joanna, Chlopicki Stefan, Kaczor Agnieszka
Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, Poland.
Analyst. 2021 Jan 4;146(1):270-276. doi: 10.1039/d0an01868f.
Perivascular adipose tissue (PVAT) regulates vascular function and represents a novel therapeutic target in vascular diseases. In this work, a new approach based on fiber-optic Raman spectroscopy and spectral modelling was used to characterize the chemical content of the PVAT of the internal mammary artery (IMA) of patients with advanced coronary atherosclerosis (n = 10) undergoing coronary bypass surgery. Our results showed a high degree of lipid unsaturation and low carotenoid content in the PVAT of the IMA of patients with more advanced coronary artery disease. Moreover, the spectral modelling of the IMA's PVAT composition indicated that glyceryl trioleate was a major PVAT lipid and for patients with relatively low levels of β-carotene, it was accompanied by arachidonic acid and glyceryl trilinolenate. In summary, our proof-of-concept study suggests that carotenoid content and lipid unsaturation degree may reflect the PVAT functional status and a Raman-based assessment of the PVAT of the IMA could prove useful as a novel diagnostic tool to rapidly define the PVAT phenotype in a grafted artery in patients undergoing coronary bypass.
血管周围脂肪组织（PVAT）调节血管功能，是血管疾病中的一个新的治疗靶点。在这项研究中，一种基于光纤拉曼光谱和光谱建模的新方法被用于表征接受冠状动脉搭桥手术的晚期冠状动脉粥样硬化患者（n = 10）的乳内动脉（IMA）的PVAT的化学组成。我们的结果显示，在冠状动脉疾病更严重的患者的IMA的PVAT中，脂质不饱和度高且类胡萝卜素含量低。此外，IMA的PVAT组成的光谱建模表明，三油酸甘油酯是PVAT的主要脂质，对于β-胡萝卜素水平相对较低的患者，它还伴有花生四烯酸和三亚麻酸甘油酯。总之，我们的概念验证研究表明，类胡萝卜素含量和脂质不饱和度可能反映PVAT的功能状态，基于拉曼光谱对IMA的PVAT进行评估可能是一种有用的新型诊断工具，可快速确定接受冠状动脉搭桥手术患者移植动脉中PVAT的表型。
