Universidade Santa Úrsula - USU Laboratório de Inovações Tecnológicas no Ensino em Saúde - LITES Botafogo RJ Brasil Laboratório de Inovações Tecnológicas no Ensino em Saúde - LITES, Universidade Santa Úrsula - USU, Botafogo, RJ, Brasil.
Universidade Federal do Espírito Santo - UFES Programa de Pós-Graduação em Ciências Fisiológicas Departamento de Ciências Fisiológicas Vitória ES Brasil Departamento de Ciências Fisiológicas, Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Espírito Santo - UFES, Vitória, ES, Brasil.
Braz J Cardiovasc Surg. 2020 Oct 1;35(5):824-830. doi: 10.21470/1678-9741-2019-0255.
To investigate the cardiovascular effects produced by transthoracic application of low-intensity pulsed ultrasound therapy (LIPUST).
Three-month-old male Wistar rats (± 300 g, N=16) were randomly allocated in two groups, namely SHAM (control group, faked procedures) and UST (animals treated with LIPUST). These animals, under anesthesia, were instrumented (femoral artery and vein catheterization) for hemodynamic recordings (mean blood pressure [MBP], heart rate [HR]) and blood biochemical profile (lipids, creatine kinase-myocardial band [CK-MB]). Then, LIPUST (spatial average-temporal average [ISATA] 1-MHz, power 0.1 to 1.2 W/cm2, pulsed 2:8 ms, cycle at 30%, for three minutes) was applied to animals from the UST group, externally to their thorax. SHAM animals were equally manipulated, but without application of ultrasound energy. After the hemodynamic and biochemical measurements, animals were sacrificed, and their hearts were mounted in a Langendorff apparatus for coronary reactivity evaluation. Standard histology techniques were employed to analyze the hearts.
LIPUST application caused statistically significant reductions in MBP (92±4 vs. 106±1 mmHg) and HR (345±14 vs. 380±17 rpm) when compared with SHAM procedures. UST rats exhibited higher CK-MB levels (318±55 vs. 198±26 U/dL) and lower plasma triglycerides levels (38±7 vs. 70±10 mg/dL) than SHAM animals. Coronary reactivity was not significantly changed by LIPUST. Cardiac histopathology showed an increase in capillary permeability in treated animals when compared with SHAM animals.
Noninvasive LIPUST induces significant metabolic and hemodynamic changes, including intensity-dependent bradycardia and hypotension, indicating a possible therapeutic effect for cardiac events.
研究经胸应用低强度脉冲超声治疗（LIPUST）产生的心血管效应。
将 3 月龄雄性 Wistar 大鼠（±300g，N=16）随机分为两组，即 SHAM（对照组，假手术）和 UST（接受 LIPUST 治疗的动物）。这些动物在麻醉下进行了（股动脉和静脉置管）以进行血流动力学记录（平均血压[MBP]、心率[HR]）和血液生化特征（脂质、肌酸激酶-心肌带[CK-MB]）。然后，将 LIPUST（空间平均-时间平均[ISATA]1MHz、功率 0.1 至 1.2W/cm2、脉冲 2:8ms、周期 30%，持续三分钟）应用于 UST 组动物的胸部。SHAM 动物也进行了同样的操作，但没有应用超声能量。进行血流动力学和生化测量后，处死动物，将其心脏安装在 Langendorff 仪器中进行冠状动脉反应性评估。采用标准组织学技术分析心脏。
与 SHAM 操作相比，LIPUST 应用导致 MBP（92±4 对 106±1mmHg）和 HR（345±14 对 380±17rpm）显著降低。UST 大鼠的 CK-MB 水平（318±55 对 198±26U/dL）较高，血浆甘油三酯水平（38±7 对 70±10mg/dL）较低。LIPUST 并未显著改变冠状动脉反应性。心脏组织病理学显示，与 SHAM 动物相比，治疗动物的毛细血管通透性增加。
非侵入性 LIPUST 会引起明显的代谢和血流动力学变化，包括强度依赖性心动过缓和低血压，表明其可能对心脏事件有治疗作用。
