Ionera Technologies GmbH, Freiburg, Germany.
Nanion Technologies GmbH, Munich, Germany.
Methods Mol Biol. 2021;2188:67-92. doi: 10.1007/978-1-0716-0818-0_4.
Artificial lipid bilayers have been used for several decades to study channel-forming pores and ion channels in membranes. Until recently, the classical two-chamber setups have been primarily used for studying the biophysical properties of pore forming proteins. Within the last 10 years, instruments for automated lipid bilayer measurements have been developed and are now commercially available. This chapter focuses on protein purification and reconstitution of channel-forming proteins into lipid bilayers using a classic setup and on the commercially available systems, the Orbit mini and Orbit 16.
人工脂质双层已被使用了几十年，用于研究膜中的通道形成孔和离子通道。直到最近，经典的两室设置主要用于研究孔形成蛋白的生物物理特性。在过去的 10 年中，已经开发出用于自动化脂质双层测量的仪器，并且现在已经商业化。本章重点介绍使用经典设置和商业上可用的系统（Orbit mini 和 Orbit 16）将通道形成蛋白纯化并重组到脂质双层中的方法。
