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采用顶空固相微萃取-气相色谱/质谱联用结合电子鼻和电子舌对哈尔滨红肠的风味特征进行分析。

Characterization of selected Harbin red sausages on the basis of their flavour profiles using HS-SPME-GC/MS combined with electronic nose and electronic tongue.

机构信息

College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.

Shimadzu (China) Co., LTD., Shenyang, Liaoning 110000, China.

出版信息

Meat Sci. 2021 Feb;172:108345. doi: 10.1016/j.meatsci.2020.108345. Epub 2020 Oct 14.

DOI:10.1016/j.meatsci.2020.108345
PMID:33120175
Abstract

In this study, volatile profiles and taste properties of Harbin red sausages prepared with traditional and conventional processing methods were evaluated using solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC/MS) coupled with electronic nose (E-nose) and electronic tongue (E-tongue). Four varieties of traditional sausages and four varieties of conventional sausages were selected. A total of 131 volatiles were identified, of which 50 were found in all sausages, and of which 77 were significantly different between traditional sausages and conventional sausages. The total contents of aldehydes (211.32 μg/kg), ketones (404.28 μg/kg), phenols (1795.40 μg/kg) and furans (928.73 μg/kg) in the traditional sausages were significantly higher than those in the conventional sausages (P < 0.05). The results of GC/MS coupled with E-nose based on principal component analysis could discriminate the sausages well, but the E-tongue could not clearly distinguish them. The results of partial least square regression (P < 0.05, Q = 0.659) indicated that there was a high correlation between the E-nose sensors and volatiles of Harbin red sausages.

摘要

本研究采用固相微萃取气质联用(SPME-GC/MS)结合电子鼻(E-nose)和电子舌(E-tongue)技术,对采用传统和常规加工方法制备的哈尔滨红肠的挥发性成分和口感特性进行了评价。选择了 4 种传统香肠和 4 种常规香肠。共鉴定出 131 种挥发性物质,其中 50 种存在于所有香肠中,其中 77 种在传统香肠和常规香肠之间存在显著差异。传统香肠中醛类(211.32μg/kg)、酮类(404.28μg/kg)、酚类(1795.40μg/kg)和呋喃类(928.73μg/kg)的总含量明显高于常规香肠(P<0.05)。基于主成分分析的 GC/MS 与 E-nose 的结合结果能够很好地区分香肠,但 E-tongue 无法清晰地区分它们。偏最小二乘回归(P<0.05,Q=0.659)的结果表明,E-nose 传感器与哈尔滨红肠挥发性成分之间存在高度相关性。

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