Azab Ehab, Hegazy Ahmad K, Gobouri Adil A, Elkelish Amr
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Sharkia, Egypt.
Biology (Basel). 2020 Oct 27;9(11):362. doi: 10.3390/biology9110362.
The excessive use of herbicides is a major cause of many environmental problems. The use of isoproturon herbicide as a weed controller has been a common practice globally. Phytoremediation technology can help in cleaning up polluted areas. In this paper the ability of transgenic plants in the phytoremediation of isoproturon herbicides has been investigated. We tested the capability of P450-1A2 overexpression on the detoxification and degradation of isoproturon. We explored the toxic effect of isoproturon on the plant phenotypic characteristics, including the primary root length, rosette diameter, and fresh, dry weight for transgenic and wild type . The results revealed that no morphological changes appeared on transgenic plants with a high tolerance to isoproturon herbicide applications either via foliar spraying or supplementation of the growth medium. Deleterious effects were observed on the morphological characteristics of plants of the wild type grown in soil under different treatments with isoproturon. The transgenic plants exhibited a vigorous growth even at high doses of isoproturon treatments. In contrast, the growth of the wild type was significantly impaired with doses above 50 µM isoproturon. The transgenic plants expressing P450-1A2 were able to metabolize the phenylurea herbicide isoproturon. Therefore, this method can be determined as a potential bioremediation agent.
除草剂的过度使用是许多环境问题的主要原因。使用异丙隆除草剂作为杂草控制剂在全球范围内是一种常见做法。植物修复技术有助于清理污染区域。本文研究了转基因植物对异丙隆除草剂的植物修复能力。我们测试了P450-1A2过表达对异丙隆解毒和降解的能力。我们探究了异丙隆对植物表型特征的毒性作用，包括转基因和野生型植物的初生根长度、莲座直径以及鲜重和干重。结果表明，通过叶面喷施或在生长培养基中添加异丙隆除草剂，对异丙隆具有高耐受性的转基因植物未出现形态变化。在不同异丙隆处理的土壤中生长的野生型植物的形态特征出现了有害影响。即使在高剂量的异丙隆处理下，转基因植物仍表现出旺盛生长。相比之下，当异丙隆剂量高于50 µM时，野生型植物的生长受到显著损害。表达P450-1A2的转基因植物能够代谢苯基脲除草剂异丙隆。因此，该方法可被确定为一种潜在的生物修复剂。
