Systems Biology Research Center, School of Bioscience, University of Skövde, P.O. Box 408, SE-541 28 Skövde, Sweden.
Systems Biology Research Center, School of Bioscience, University of Skövde, P.O. Box 408, SE-541 28 Skövde, Sweden.
J Plant Physiol. 2017 Nov;218:121-126. doi: 10.1016/j.jplph.2017.08.001. Epub 2017 Aug 4.
We have cloned, characterized and transformed the AtACR2 gene (arsenic reductase 2) of Arabidopsis thaliana into the genome of tobacco (Nicotiana tabacum, var Sumsun). Our results revealed that the transgenic tobacco plants are more tolerant to arsenic than the wild type ones. These plants can grow on culture medium containing 200μM arsenate, whereas the wild type can barely survive under this condition. Furthermore, when exposed to 100μM arsenate for 35days the amount of arsenic accumulated in the shoots of transgenic plants was significantly lower (28μg/g d wt.) than that found in the shoots of non-transgenic controls (40μg/g d wt.). However, the arsenic content in the roots of transgenic plants was significantly higher (2400μg/g d. wt.) than that (2100μg/g d. wt.) observed in roots of wild type plants. We have demonstrated that Arabidopsis thaliana AtACR2 gene is a potential candidate for genetic engineering of plants to develop new crop cultivars that can be grown on arsenic contaminated fields to reduce arsenic content of the soil and can become a source of food containing no arsenic or exhibiting substantially reduced amount of this metalloid.
我们已经将拟南芥的 AtACR2 基因（砷还原酶 2）克隆、鉴定并转化到烟草（Nicotiana tabacum，var Sumsun）的基因组中。我们的结果表明，转基因烟草植物比野生型植物更能耐受砷。这些植物可以在含有 200μM 砷酸盐的培养基上生长，而野生型植物在这种条件下几乎无法存活。此外，当暴露于 100μM 砷酸盐 35 天时，转基因植物地上部分积累的砷含量明显低于非转基因对照（28μg/g d wt.）（40μg/g d wt.）。然而，转基因植物根系中的砷含量明显高于野生型植物（2400μg/g d. wt.）（2100μg/g d. wt.）。我们已经证明，拟南芥 AtACR2 基因是植物遗传工程的潜在候选基因，可以培育出能够在砷污染农田上生长的新作物品种，以降低土壤中的砷含量，并成为不含砷或含砷量显著降低的食物来源。
