Nishimaki-Mogami T, Yamanaka H, Mizugaki M
Pharmaceutical Institute, Tohoku University, Miyagi.
J Biochem. 1987 Aug;102(2):427-32. doi: 10.1093/oxfordjournals.jbchem.a122070.
The activity of acetyl-CoA-dependent chain elongation of fatty acids in Escherichia coli was enhanced when the organism was grown on oleic acid as the sole carbon source, but not detected when grown on glucose. Antibodies raised against fatty acid oxidation complex of E. coli inhibited both the reaction catalyzed by crotonase and the chain elongation in a similar manner, showing that the oxidation complex participates in the chain elongation. The activities of condensation and the activities of NADH- and NADPH-dependent 3-ketoacyl reduction in the cell-free extract were precipitated by antibodies to the complex in parallel with those of 3-ketoacyl-CoA thiolase and crotonase. These results together with the presence of NADPH-dependent trans-2-enoyl-CoA reductase in E. coli (Mizugaki, et al. (1982) Chem. Pharm. Bull. 30, 2503-2511) indicate that the acetyl-CoA-dependent chain elongation of fatty acids in E. coli occurs by the reversal of fatty acid oxidation other than the step of enoyl reduction.
当大肠杆菌以油酸作为唯一碳源生长时，其脂肪酸乙酰辅酶A依赖性链延长活性增强，但以葡萄糖为碳源生长时未检测到该活性。针对大肠杆菌脂肪酸氧化复合物产生的抗体以类似方式抑制了巴豆酸酶催化的反应和链延长，表明氧化复合物参与了链延长。无细胞提取物中缩合活性以及NADH和NADPH依赖性3-酮酰基还原活性，与3-酮酰基辅酶A硫解酶和巴豆酸酶的活性一样，被针对该复合物的抗体沉淀。这些结果连同大肠杆菌中存在NADPH依赖性反式-2-烯酰辅酶A还原酶（水垣等人，（1982年）《化学与药学通报》30，2503 - 2511）表明，大肠杆菌中脂肪酸的乙酰辅酶A依赖性链延长是通过脂肪酸氧化的逆反应发生的，烯酰还原步骤除外。
