Bickel M, Amstad P, Tsuda H, Sulis C, Asofsky R, Mergenhagen S E, Pluznik D H
Laboratory of Microbiology and Immunology, National Institute of Dental Research, Bethesda, Maryland 20892.
J Immunol. 1987 Nov 1;139(9):2984-8.
The present study was undertaken to elucidate whether B cell lymphoma and hybridoma cell lines can be stimulated by lipopolysaccharides (LPS) or by antibodies against immunoglobulin M (IgM) to produce granulocyte-macrophage colony-stimulating factor (GM-CSF). GM-CSF activity was assayed on the basophil/mast cell line PT-18 which is GM-CSF- and interleukin 3-dependent. Antibodies against murine recombinant GM-CSF were used to identify the colony-stimulating factor activity present in the supernatants of the stimulated B cell lines. When these cell lines were stimulated with LPS, two of five lymphoma and five of six hybridoma lines produced GM-CSF. Two cell lines, the B cell lymphoma M12.4.1 and the hybridoma TH2.2, were analyzed more extensively under serum-free conditions. In these two cell lines, the production of GM-CSF was dependent on the dose of LPS used and time of exposure. Antibodies against IgM stimulated the TH2.2 (IgM+) but not the M12.4.1 (IgM-) cells to produce GM-CSF. Northern blot analysis of the M12.4.1 and TH2.2 cells showed that mRNA of GM-CSF can be detected in LPS-stimulated but not in unstimulated cells. Our data show that transformed B cells can be stimulated to produce GM-CSF. The present data and previous studies on GM-CSF production by normal bone marrow-derived B cells suggest a possible participation of B cells in granulopoiesis.
本研究旨在阐明B细胞淋巴瘤和杂交瘤细胞系是否能被脂多糖（LPS）或抗免疫球蛋白M（IgM）抗体刺激以产生粒细胞-巨噬细胞集落刺激因子（GM-CSF）。GM-CSF活性在依赖GM-CSF和白细胞介素3的嗜碱性粒细胞/肥大细胞系PT-18上进行测定。使用抗小鼠重组GM-CSF的抗体来鉴定受刺激的B细胞系上清液中存在的集落刺激因子活性。当用LPS刺激这些细胞系时，五个淋巴瘤细胞系中的两个以及六个杂交瘤细胞系中的五个产生了GM-CSF。在无血清条件下对两个细胞系，即B细胞淋巴瘤M12.4.1和杂交瘤TH2.2进行了更广泛的分析。在这两个细胞系中，GM-CSF的产生取决于所用LPS的剂量和暴露时间。抗IgM抗体刺激TH2.2（IgM阳性）细胞而非M12.4.1（IgM阴性）细胞产生GM-CSF。对M12.4.1和TH2.2细胞的Northern印迹分析表明，GM-CSF的mRNA可在LPS刺激的细胞中检测到，而在未刺激的细胞中未检测到。我们的数据表明，转化的B细胞可被刺激产生GM-CSF。本数据以及先前关于正常骨髓来源B细胞产生GM-CSF的研究表明，B细胞可能参与粒细胞生成。
