MacPhee M J, Rode H, Broadhead M, Christou N V, Meakins J L, Gordon J
Department of Surgery, McGill University, Montreal, Quebec, Canada.
J Immunol Methods. 1987 Nov 5;103(2):267-73. doi: 10.1016/0022-1759(87)90299-7.
A method is described for the recovery of cells involved in the delayed-type hypersensitivity (DTH) reaction in man. A small plastic chamber was placed over DTH sites from which the stratum corneum had been removed by tape stripping. The chambers were then filled with serum. Analysis of cells harvested at 24 and 48 h has shown an antigen-dependent increment in mononuclear and polymorphonuclear cells in donors with a DTH response. T cell clones were established from the recovered cells; these will provide a useful adjunct to a functional analysis of cells participating in the DTH reaction.
本文描述了一种用于回收参与人类迟发型超敏反应（DTH）的细胞的方法。将一个小塑料腔室放置在通过胶带剥离已去除角质层的DTH部位上。然后向腔室中填充血清。对在24小时和48小时收获的细胞进行分析表明，具有DTH反应的供体中单核细胞和多形核细胞的数量出现了抗原依赖性增加。从回收的细胞中建立了T细胞克隆；这些克隆将为参与DTH反应的细胞的功能分析提供有用的辅助手段。
