Zhang Xing-Xing, Wang Chao-Chao, Wang Yao, Xu Le-Zhong, Wu Peng
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou 215009, China.
Huan Jing Ke Xue. 2020 Aug 8;41(8):3715-3724. doi: 10.13227/j.hjkx.202001216.
To explore the feasibility of the rapid start-up of partial denitrification and the stable accumulation of NO-N in different waste sludge sources, three identical SBR reactors (S1, S2, and S3) were inoculated respectively with sludge discharged from a laboratory municipal wastewater denitrifying phosphorus removal system, surplus sludge from a municipal wastewater treatment plant, and river sediment sludge. The characteristics of the partial denitrification start-up and NO-N accumulation were compared, and the partial denitrification activity of the system or NO-N→NO-N transformation performance were investigated by analyzing the characteristics of the functional bacteria genera of the reactor from the perspective of microbiology. The results showed that all three SBR partial denitrification reactors could be launched successfully in a short time with sodium acetate as the sole carbon source, under a high alkalinity, and by using a suitable COD/NO-N ratio. The average NO-N→NO-N transformation ratio of the system was ranked as:S1 > S2 > S3 (75.92% > 73.36% > 69.90%). It was found that S1 and S2 had different degrees of partial denitrification performance deterioration under a continuous low temperature, but that S3 could maintain a good NO-N accumulation performance. High throughput sequencing showed that Proteobacteria and Bacteroidetes were dominant in the partial denitrification system, and that the abundance of was significantly different in the three PD reactors:S3 > S1 > S2 (25.09% > 4.71% > 3.60%), thus indicating that S3 had stable and efficient NO-N accumulation performance and that a high abundance of might play a significant role in maintaining low temperature partial denitrification activity.
为探究不同废弃污泥来源下部分反硝化快速启动及亚硝酸盐氮（NO-N）稳定积累的可行性，分别向三个相同的序批式反应器（S1、S2和S3）接种来自实验室城市污水反硝化除磷系统排放的污泥、城市污水处理厂的剩余污泥以及河流底泥污泥。比较了部分反硝化启动及NO-N积累的特性，并从微生物学角度分析反应器功能细菌属的特征，研究系统的部分反硝化活性或NO-N→NO-N转化性能。结果表明，以乙酸钠为唯一碳源，在高碱度及合适的COD/NO-N比条件下，三个SBR部分反硝化反应器均可在短时间内成功启动。系统的平均NO-N→NO-N转化率排序为：S1＞S2＞S3（75.92%＞73.36%＞69.90%）。发现S1和S2在持续低温下部分反硝化性能有不同程度的恶化，但S3能保持良好的NO-N积累性能。高通量测序表明，变形菌门和拟杆菌门在部分反硝化系统中占主导，且三个部分反硝化（PD）反应器中该菌门的丰度差异显著：S3＞S1＞S2（25.09%＞4.71%＞3.60%），这表明S3具有稳定高效的NO-N积累性能，且该菌门的高丰度可能在维持低温部分反硝化活性中起重要作用。
