Lu Ming-Yu, Li Xiang, Huang Yong, Yin Ji-Qiang, Fang Wen-Ye
School of Environment Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China.
Huan Jing Ke Xue. 2020 Oct 8;41(10):4644-4652. doi: 10.13227/j.hjkx.202003002.
To promote the application of ANAMMOX process in landfill leachate treatment, a pilot reactor based on the ANAMMOX process was established at a landfill site. In this paper, we aim to further analyze the influence of different coupling modes of denitrification (DN) and partial nitrification and ANAMMOX (PN-ANAMMOX) on the diversity of microbial community. The DN+(PN-ANAMMOX) process could effectively treat the mature leachate. However, with an increase in organic matter in the influent, the oxygen demand of PN zone increased, and the enrichment of Nitrosomonadaceae in the PN zone was limited. The lack of substrate supply for ANAMMOX zone further limited the enrichment of Brocadiaceae as well; thus, the total nitrogen removal rate (TNRR) remained at 0.44 kg ·(m ·d). In the DN-(PN-ANAMMOX) process, Saprospiraceae with denitrifying ability was enriched in the DN zone, and the organic matter was gradually degraded and removed; thus, a good low-carbon environment was provided for the subsequent PN-ANAMMOX process. Nitrosomonadaceae and Brocadiaceae were enriched in the functional zones, and the TNRR and total nitrogen removal efficiency (TNRE) of the DN-(PN-ANAMMOX) were further elevated to 0.55 kg ·(m ·d) and 94.65%, respectively. Moreover, the direct treatment of mature leachate with 2233 mg ·L NH-N and 2712 mg ·L COD was finally realized. In addition, was better adapted to leachate and high substrate concentration wastewater, and it became the dominant genus in the ANAMMOX zone.
为促进厌氧氨氧化工艺在垃圾渗滤液处理中的应用，在某垃圾填埋场建立了基于厌氧氨氧化工艺的中试反应器。本文旨在进一步分析反硝化（DN）与部分硝化和厌氧氨氧化（PN-ANAMMOX）不同耦合模式对微生物群落多样性的影响。DN+(PN-ANAMMOX)工艺能够有效处理成熟渗滤液。然而，随着进水有机物含量增加，PN区的需氧量增加，PN区亚硝化单胞菌科的富集受到限制。厌氧氨氧化区缺乏底物供应也进一步限制了布罗卡德氏菌科的富集；因此，总氮去除率（TNRR）维持在0.44 kg·(m³·d)。在DN-(PN-ANAMMOX)工艺中，具有反硝化能力的腐螺旋菌科在DN区富集，有机物逐渐降解去除；因此，为后续的PN-ANAMMOX工艺提供了良好的低碳环境。亚硝化单胞菌科和布罗卡德氏菌科在功能区富集，DN-(PN-ANAMMOX)工艺的TNRR和总氮去除效率（TNRE）分别进一步提高到0.55 kg·(m³·d)和94.65%。此外，最终实现了对氨氮为2233 mg·L、化学需氧量为2712 mg·L的成熟渗滤液的直接处理。此外， 对渗滤液和高底物浓度废水具有更好的适应性，成为厌氧氨氧化区的优势菌属。 （注：原文中“ In addition, was better adapted to leachate and high substrate concentration wastewater, and it became the dominant genus in the ANAMMOX zone.”这里的“ ”处原文缺失内容）
