Facultad de Farmacia, Departamento de Química en Ciencias Farmacéuticas, Universidad Complutense de Madrid, Spain.
Phys Chem Chem Phys. 2020 Nov 21;22(43):25165-25176. doi: 10.1039/d0cp03816d. Epub 2020 Oct 30.
This work reports the experimental measurements of solvent acidity (SA), basicity (SB), and solvent dipolarity and polarizability (SPP) for water solutions with urea (U) and its molecular derivatives, monomethyl-urea (MU), 1,3-dimethyl-urea (DMU) and tetramethyl-urea (TMU). These solvatochromic parameters are applied to understanding the variation of indexes of refraction and densities and other physico-chemical properties reported for these solutions. These properties are well correlated to the SA, SB, and SPP solvent parameters of these solutions. As a result, from the characterization of the physico-chemical properties, one can infer that urea and its molecular derivatives are mainly modifiers in the structure of liquid water. The solvatochromic parameters indicate the possible existence of different mechanisms in the denaturation process of proteins in these urea/water solutions.
本工作报道了水溶剂中脲(U)及其分子衍生物(单甲基脲(MU)、1,3-二甲基脲(DMU)和四甲基脲(TMU))的溶剂酸度(SA)、碱度(SB)和溶剂偶极矩和极化率(SPP)的实验测量结果。这些溶剂参数被应用于理解这些溶液的折射率和密度以及其他物理化学性质的变化。这些性质与这些溶液的 SA、SB 和 SPP 溶剂参数很好地相关。因此,通过对物理化学性质的特征描述,可以推断出脲及其分子衍生物主要是液态水中结构的修饰剂。溶剂参数表明在这些脲/水溶液中蛋白质的变性过程中可能存在不同的机制。