Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664, Warsaw, Poland.
Department of Physics and Biophysics, Institute of Biology, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-787, Warsaw, Poland.
Chembiochem. 2021 Mar 16;22(6):1020-1029. doi: 10.1002/cbic.202000656. Epub 2020 Nov 26.
Amphiphilic antimicrobial polymers display activity against the outer bacterial cell membrane, triggering various physiological effects. We investigated the regulation of ion transport across the lipid bilayer to understand differences in biological activity for a series of amphiphilic polymethyloxazoline - polyethyleneimine copolymers. The results confirmed that the tested structures were able to increase the permeability of the lipid bilayer (LB) membrane or its rupture. Black lipid membrane (BLM) experiments show that the triggered conductance profile and its character is strongly correlated with the polymer structure and zeta potential. The polymer exhibiting the highest antimicrobial activity promotes ion transport by using a unique mechanism and step-like characteristics with well-defined discreet openings and closings. The molecule was incorporated into the membrane in a reproducible way, and the observed channel-like activity could be responsible for the antibacterial activity of this molecule.
两亲性抗菌聚合物对细菌外细胞膜表现出活性，引发各种生理效应。我们研究了离子跨脂质双层的传输调节，以了解一系列亲水性聚甲基恶唑啉-聚乙烯亚胺共聚物的生物活性差异。结果证实，所测试的结构能够增加脂质双层（LB）膜的通透性或使其破裂。黑脂质膜（BLM）实验表明，触发的电导谱及其特征与聚合物结构和zeta 电位密切相关。表现出最高抗菌活性的聚合物通过使用独特的机制和阶跃特性促进离子传输，具有明确定义的离散开口和闭合。该分子以可重复的方式掺入膜中，观察到的类似通道的活性可能是该分子具有抗菌活性的原因。
