含有光不稳定基团的甲硫氨酰 - tRNA合成酶底物类似物。
Analogs of methionyl-tRNA synthetase substrates containing photolabile groups.
作者信息
Wetzel R, Söll D
出版信息
Nucleic Acids Res. 1977;4(5):1681-94. doi: 10.1093/nar/4.5.1681.
Abstract
Three photolabile analogs of substrates of methionyl-tRNA synthetase were synthesized. In one, the 4-thiouridine at the 8 position of E. coli tRNAfMet was alkylated with [14C]p-azidobromoacetanilide. In the second, [14C]p-azidobenzoic acid hydrazide was condensed with the 3'-terminal dialdehyde of periodate-oxidized Escherichia coli tRNAfMet. The modified tRNAs could be purified by chromatography on benzoylated DEAE-cellulose. The third photolabile compound was [3H]methioninyl-8-azido-adenosine 5'-phosphate, an analog of the methionyl adenylate intermediate in the aminoacylation reaction. Irradiation of each of these compounds in the presence of equimolar amounts of E. coli methionyl-tRNA synthetase of micrometer concentrations gave 5-15% crosslinking.
摘要
合成了甲硫氨酰 - tRNA合成酶底物的三种光不稳定类似物。第一种是用[¹⁴C]对叠氮基溴乙酰苯胺将大肠杆菌起始tRNA（tRNAfMet）第8位的4 - 硫尿苷烷基化。第二种是将[¹⁴C]对叠氮基苯甲酸酰肼与高碘酸盐氧化的大肠杆菌起始tRNA的3' - 末端二醛缩合。修饰后的tRNA可通过在苯甲酰化DEAE - 纤维素上进行色谱纯化。第三种光不稳定化合物是[³H]甲硫氨酰 - 8 - 叠氮腺苷5'-磷酸，是氨酰化反应中甲硫氨酰腺苷酸中间体的类似物。在存在微摩尔浓度的等摩尔量大肠杆菌甲硫氨酰 - tRNA合成酶的情况下，对这些化合物中的每一种进行辐照，可产生5 - 15%的交联。
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Nucleic Acids Res. 1977;4(5):1681-94. doi: 10.1093/nar/4.5.1681.
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