抗17α-羟孕酮单克隆抗体的制备与特性鉴定
Production and characterization of monoclonal antibodies to 17 alpha-hydroxyprogesterone.
作者信息
Sawada J, Terao T, Itoh S, Maeda M, Tsuji A, Hosoda H, Nambara T
机构信息
Division of Radiochemistry, National Institute of Hygienic Sciences, Tokyo, Japan.
出版信息
J Steroid Biochem. 1987 Oct;28(4):405-10. doi: 10.1016/0022-4731(87)91058-2.
Hybridoma clones producing antibodies to 17 alpha-hydroxyprogesterone (17-OHP) were established by using a 17-OHP-bovine serum albumin conjugate as an immunogen. Six representative IgG-class monoclonal antibodies of high affinity (10(8)-10(9) M-1) showed differential reactivities with several structurally related steroids. Two enzyme immunoassay (EIA) systems (fluorescence EIA and micro-EIA) for 17-OHP using OHP 4B2.2.3, which showed the lowest cross-reactivity with other steroids, were established. The micro-EIA system was shown to be applicable to the mass-screening of congenital adrenal hyperplasia.
以17α-羟孕酮（17-OHP）-牛血清白蛋白偶联物作为免疫原，建立了产生抗17α-羟孕酮抗体的杂交瘤克隆。六种具有高亲和力（10⁸ - 10⁹ M⁻¹）的代表性IgG类单克隆抗体对几种结构相关的甾体激素表现出不同的反应性。使用与其他甾体激素交叉反应性最低的OHP 4B2.2.3，建立了两种用于17-OHP的酶免疫分析（EIA）系统（荧光EIA和微量EIA）。微量EIA系统被证明适用于先天性肾上腺皮质增生症的大规模筛查。
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