Wei Qiaoling, Zhuang Xiaonan, Fan Jiawen, Jiang Rui, Chang Qing, Xu Gezhi, Yu Zhiqiang
Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai, China.
Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai, China.
Cytokine. 2021 Jan;137:155308. doi: 10.1016/j.cyto.2020.155308. Epub 2020 Oct 28.
To determine the concentrations of vitreous proinflammatory cytokines and angiogenesis-related growth cytokines in highly myopic (HM) patients and controls.
Vitreous humor (VH) was obtained from patients during vitrectomy for rhegmatogenous retinal detachment (RRD), myopic retinoschisis (MRS), idiopathic epiretinal membrane (ERM), or macular hole (MH). High myopia was defined as an axial length (AL) of ≥26.0 mm and a spherical equivalent refractive error more negative than -6.0 D. A multiplex fluorescent-bead-based immunoassay was employed to measure the levels of 29 designated cytokines. The results were compared across groups.
Seventy-eight VH samples were collected from 78 patients (36 HM versus 42 controls). Vascular endothelial growth factor (VEGF) was significantly higher in the VH samples from HM patients than in those from the controls. Five inflammation-related factors, interferon γ (IFN-γ), interleukin 6 (IL6), IFN-γ-induced protein 10 (IP-10), eotaxin, and macrophage inflammatory protein 1α (MIP-1α), were significantly higher in the HM group than in the control group. The vitreous concentrations of well-known angiogenic growth factors monocyte chemoattractant protein 1 (MCP1) and IL5 were significantly elevated in the VH samples from HM patients.
Proinflammatory cytokines and angiogenic growth factors were elevated in the VH of HM patients, suggesting that an elevated inflammatory status and higher levels of angiogenic factors are present in eyes with HM.
测定高度近视（HM）患者和对照组玻璃体内促炎细胞因子和血管生成相关生长细胞因子的浓度。
在玻璃体切除术治疗孔源性视网膜脱离（RRD）、近视性视网膜劈裂（MRS）、特发性视网膜前膜（ERM）或黄斑裂孔（MH）期间，从患者获取玻璃体液（VH）。高度近视定义为眼轴长度（AL）≥26.0毫米且等效球镜屈光不正低于-6.0 D。采用基于多重荧光微珠的免疫测定法测量29种指定细胞因子的水平。对各组结果进行比较。
从78例患者（36例HM患者与42例对照）中收集了78份VH样本。HM患者的VH样本中血管内皮生长因子（VEGF）显著高于对照组。HM组中5种炎症相关因子，即干扰素γ（IFN-γ）、白细胞介素6（IL6）、IFN-γ诱导蛋白10（IP-10）、嗜酸性粒细胞趋化因子和巨噬细胞炎性蛋白1α（MIP-1α）显著高于对照组。HM患者的VH样本中著名的血管生成生长因子单核细胞趋化蛋白1（MCP1）和IL5的玻璃体内浓度显著升高。
HM患者的VH中促炎细胞因子和血管生成生长因子升高，提示HM眼中存在炎症状态升高和血管生成因子水平升高的情况。
