阿尔茨海默病中应激相关代谢和细胞反应通路所调控的循环 hsa-miR-494-3p 和 hsa-miR-661 的作用研究。
The Highlighted Roles of Metabolic and Cellular Response to Stress Pathways Engaged in Circulating hsa-miR-494-3p and hsa-miR-661 in Alzheimer's Disease.
机构信息
Division of Genetics, Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan 8174673441, Iran.
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
出版信息
Iran Biomed J. 2021 Jan;25(1):62-7. doi: 10.29252/ibj.25.1.62. Epub 2020 Aug 31.
BACKGROUND
: Among different roles of miRNAs in AD pathogenesis, hsa-miR-494-3p and hsa-miR-661 functions are poorly understood.
METHODS
: To obtain the gene targets, gene networks, gene ontology, and enrichment analysis of the two miRNAs, some web servers were utilized. Furthermore, the expressions of these miRNAs were analyzed by qRT-PCR in 36 blood sera, including 18 Alzheimer’s patients and 18 healthy individuals.
RESULTS
: The analysis demonstrated the highlighted roles of metabolic and cellular response to stress pathways engaged in circulating hsa-miR-494-3p and hsa-miR-661 in AD. The qRT-PCR analysis showed that the downregulated expression level of hsa-miR-661 was statistically significant ( < 0.05). Also, the ROC curve of hsa-miR-661 displayed the significant AUC ( = 0.01).
CONCLUSION
: Based on our findings, the metabolic and cellular responses to stress pathways are closely connected to these two miRNAs functions. Besides, the qRT-PCR and Roc curve determined hsa-miR-661 could be as a biomarker for diagnosis or prognosis of AD patients.
背景
在 AD 发病机制中，miRNAs 有多种作用，其中 hsa-miR-494-3p 和 hsa-miR-661 的功能尚不清楚。
方法
为了获得这两种 miRNA 的基因靶标、基因网络、基因本体论和富集分析，我们利用了一些网络服务器。此外，通过 qRT-PCR 分析了 36 份血清样本（包括 18 名阿尔茨海默病患者和 18 名健康个体）中这两种 miRNA 的表达情况。
结果
分析表明，代谢和细胞应激反应途径在 AD 患者的循环 hsa-miR-494-3p 和 hsa-miR-661 中发挥着重要作用。qRT-PCR 分析显示，hsa-miR-661 的表达水平显著下调（<0.05）。此外，hsa-miR-661 的 ROC 曲线显示出显著的 AUC（=0.01）。
结论
根据我们的研究结果，代谢和细胞应激反应途径与这两种 miRNA 的功能密切相关。此外，qRT-PCR 和 Roc 曲线确定 hsa-miR-661 可以作为 AD 患者诊断或预后的生物标志物。
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