脂质膜对荧光探针光学性质的影响。

The influence of lipid membranes on fluorescent probes' optical properties.

机构信息

Biological Systems Simulation Lab, Centre of New Technologies, University of Warsaw, Banacha 2C, 02-097 Warsaw, Poland.

Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic; Department of Theoretical Chemistry and Biology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden; Theoretical Physics, Hasselt University, Agoralaan Building D, 3590 Diepenbeek, Belgium.

出版信息

Biochim Biophys Acta Biomembr. 2021 Feb 1;1863(2):183494. doi: 10.1016/j.bbamem.2020.183494. Epub 2020 Oct 29.

DOI:10.1016/j.bbamem.2020.183494

PMID:33129783

Abstract

BACKGROUND

Organic fluorophores embedded in lipid bilayers can nowadays be described by a multiscale computational approach. Combining different length and time scales, a full characterization of the probe localization and optical properties led to novel insight into the effect of the environments.

SCOPE OF REVIEW

Following an introduction on computational advancements, three relevant probes are reviewed that delineate how a multiscale approach can lead to novel insight into the probes' (non) linear optical properties. Attention is paid to the quality of the theoretical description of the optical techniques.

MAJOR CONCLUSIONS

Computation can assess a priori novel probes' optical properties and guide the analysis and interpretation of experimental data in novel studies. The properties can be used to gain information on the phase and condition of the surrounding biological environment.