Department of Rehabilitation Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Institute of Geriatrics, Guangzhou, China.
Department of Rehabilitation Medicine, Guangdong Provincial Hospital of Integrated Traditional Chinese and Western Medicine, Foshan, Guangdong, China.
Neurosci Lett. 2021 Jan 10;741:135463. doi: 10.1016/j.neulet.2020.135463. Epub 2020 Oct 29.
To compare the effects of high- (HF-EMF) and low-frequency electromagnetic fields (LF-EMF) on the proliferation and differentiation of neural stem cells (NSCs). NSCs were obtained from SD rat hippocampus and cultured in suspension and adherent differentiation media. NSCs were exposed to LF-EMF (5 m T, 50 Hz, 30 min daily), HF-EMF (maximum magnetic induction 2.5 T, 40 % MO, 50 Hz, 10 min daily) and no electromagnetic field. At 3 d, cell viability and quantity of NSCs in suspension were detected by CCK-8 assay and cell counting plate. Immunofluorescence staining and qRT-PCR were performed to detect the percentage of Tuj-1 and GFAP-positive NSCs and the expression of Tuj-1 and GFAP mRNA. The P3 NSCs were positive with Nestin and induced NSCs expressed Tuj-1, GFAP and oligodendrocyte markers (MBP). CCK-8 assay and cell counting showed that the OD value and quantity of LF-EMF group were significantly higher than those in other two groups (both P < 0.05). Compared with the control group, the OD value and quantity were significantly higher in the HF-EMF group (P < 0.05). Immunofluorescence staining and qRT-PCR revealed that the percentage of Tuj-1 positive cells and the expression of Tuj-1 mRNA of NSCs exposed to LF-EMF were the highest (both P < 0.05). The proportion of GFAP-positive NSCs and the expression of GFAP mRNA did not significantly differ among three groups (all P> 0.05). Both 50 Hz LF-EMF and HF-EMF can promote the proliferation of NSCs in vitro and LF-EMF can accelerate NSCs to differentiate into neurons.
为了比较高低频电磁场（LF-EMF 和 HF-EMF）对神经干细胞（NSCs）增殖和分化的影响。从 SD 大鼠海马中获得 NSCs 并在悬浮和贴壁分化培养基中培养。将 NSCs 暴露于 LF-EMF（5 mT，50 Hz，每天 30 分钟）、HF-EMF（最大磁感应强度 2.5 T，40%调制，50 Hz，每天 10 分钟）和无电磁场中。在第 3 天，通过 CCK-8 测定法和细胞计数板检测悬浮细胞的细胞活力和数量。通过免疫荧光染色和 qRT-PCR 检测 Tuj-1 和 GFAP 阳性 NSCs 的百分比以及 Tuj-1 和 GFAP mRNA 的表达。P3 NSCs 呈 Nestin 阳性，诱导的 NSCs 表达 Tuj-1、GFAP 和少突胶质细胞标志物（MBP）。CCK-8 测定法和细胞计数表明，LF-EMF 组的 OD 值和数量明显高于其他两组（均 P<0.05）。与对照组相比，HF-EMF 组的 OD 值和数量明显更高（P<0.05）。免疫荧光染色和 qRT-PCR 显示，暴露于 LF-EMF 的 NSCs 中 Tuj-1 阳性细胞的百分比和 Tuj-1 mRNA 的表达最高（均 P<0.05）。三组之间 GFAP 阳性 NSCs 的比例和 GFAP mRNA 的表达没有显著差异（均 P>0.05）。50 Hz LF-EMF 和 HF-EMF 均可促进 NSCs 的体外增殖，LF-EMF 可加速 NSCs 向神经元分化。
