Melbourne Dental School, The University of Melbourne, Carlton, Vic., Australia.
The Centre for Oral Health Research, The University of Melbourne, Carlton, Vic., Australia.
J Clin Periodontol. 2021 Feb;48(2):249-262. doi: 10.1111/jcpe.13399. Epub 2020 Nov 22.
T-cells are known to have a role in periodontitis, however, the effect of periodontal therapy on peripheral memory T-cells is unclear. This study evaluated variation in peripheral memory T-cells and red complex bacteria in sub-gingival plaque in patients undergoing periodontal management.
Peripheral blood mononuclear cells and sub-gingival plaque were collected from 54 periodontitis patients at baseline, 3-, 6- and 12-months post-therapy and 40 healthy controls. Periodontitis patients were divided into treatment outcome (TxO) groups based on prevalence of sites with probing depth ≥5 mm as good (<10% of sites), moderate (10-20%) or poor (>20%) at study conclusion. Naïve (T -CCR7 CD45RA ), central memory (T -CCR7 CD45RA ), effector memory (T -CCR7 CD45RA ) and effector memory T-cells re-expressing CD45RA (T -CCR7 CD45RA ) were phenotyped using flow cytometry in CD4 , CD8 , CD4 CD8 and CD4 CD8 T-cells and red complex bacteria were quantified using qPCR.
At baseline, periodontitis subjects had significantly greater mean probing depths and Porphyromonas gingivalis proportions, lower T but higher CD4 T , CD8 T , CD4 CD8 T and CD4 CD8 T cell proportions compared to health. Periodontal therapy decreased mean probing depths, P. gingivalis proportions, T and CD4 and CD8 T cells, but increased T and CD4 and CD8 T cells. The T-cell profile in the good TxO group showed therapy-related changes in CD4 T , and CD8 T and T cells, whereas, no changes were observed in the poor TxO group.
Management and the reduction in red complex bacteria were associated with changes in peripheral memory T-cells in periodontitis.
T 细胞在牙周炎中起作用，然而，牙周治疗对外周记忆 T 细胞的影响尚不清楚。本研究评估了牙周治疗后患者外周记忆 T 细胞和龈下菌斑中红色复合体细菌的变化。
从 54 名牙周炎患者、40 名健康对照者的基线、治疗后 3、6 和 12 个月收集外周血单核细胞和龈下菌斑。根据研究结束时探诊深度≥5mm 的位点百分比（<10%为好，10-20%为中，>20%为差），将牙周炎患者分为治疗效果（TxO）组。采用流式细胞术在 CD4、CD8、CD4+CD8+和 CD4-CD8-T 细胞中对幼稚（T-CCR7+CD45RA+）、中央记忆（T-CCR7+CD45RA-）、效应记忆（T-CCR7-CD45RA-）和重新表达 CD45RA 的效应记忆 T 细胞（T-CCR7+CD45RA-）进行表型分析，并采用 qPCR 定量红色复合体细菌。
基线时，与健康者相比，牙周炎患者的平均探诊深度和牙龈卟啉单胞菌比例显著增加，T 细胞减少，而 CD4+T 细胞、CD8+T 细胞、CD4+CD8+T 细胞和 CD4-CD8-T 细胞比例增加。牙周治疗降低了平均探诊深度、牙龈卟啉单胞菌比例、T 细胞和 CD4+T 细胞、CD8+T 细胞，但增加了 T 细胞和 CD4+T 细胞、CD8+T 细胞。在治疗效果好的 TxO 组中，T 细胞、CD4+T 细胞和 CD8+T 细胞的治疗相关变化，而在治疗效果差的 TxO 组中则没有观察到变化。
管理和红色复合体细菌的减少与牙周炎患者外周记忆 T 细胞的变化有关。
