College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
J Dairy Sci. 2021 Jan;104(1):270-280. doi: 10.3168/jds.2020-18884. Epub 2020 Oct 31.
Today, cheese is valued because of its high nutritional value and unique characteristics. Improving the texture and flavor of cheese by selecting suitable starter cultures is an important way to promote the development of cheese industry. The effect of starter cultures on the physicochemical and textural properties and volatile compounds during the ripening of semihard goat cheese were investigated in this work. Different starter cultures-mesophilic (M) and thermophilic starters (T), Lactobacillus plantarum ssp. plantarum ATCC 14917 (Lp), a mix of the M and T starters (M1), and mix of the M, T, and Lp starters (M2)-were used in the production of the goat cheeses. Volatile compounds were determined by a solid-phase microextraction/gas chromatography-mass spectrometric (SPME/GC-MS) method. The results showed that the moisture content of cheeses produced with the 5 kinds of starter cultures decreased after maturation, whereas ash content increased. The pH values of goat cheeses decreased first and then increased during maturity, and the pH value of M2 cheese was the lowest among the cheeses. The hardness and chewiness of the cheeses increased with increasing maturity, whereas cohesiveness, springiness, and resilience showed the opposite tendency. The 60-d-old cheese made with Lp had the highest chewiness, cohesiveness, springiness, and resilience, whereas the 60-d-old cheese made with M2 had the highest hardness. A total of 53 volatile components were identified by SPME/GC-MS, and carboxylic acids, alcohols, ketones, and esters were the 4 major contributors to the characteristic flavors of the cheeses. Volatile components and their contents differed greatly among the produced cheeses. The M2 cheese contained the highest relative content of the main volatile compounds (90.10%), especially butanoic acid and acetoin. Through a comprehensive comparison of the results, we concluded that M2 cheese had a dense texture and milky flavor, and M2 is a potential starter culture candidate for the production of goat cheese.
今天,奶酪因其高营养价值和独特特性而受到重视。通过选择合适的发酵剂来改善奶酪的质地和风味是促进奶酪产业发展的重要途径。本研究考察了不同发酵剂(嗜温(M)和高温(T)发酵剂、植物乳杆菌亚种植物乳杆菌 ATCC 14917(Lp)、M 和 T 混合发酵剂(M1)和 M、T 和 Lp 混合发酵剂(M2))对半硬质山羊奶酪成熟过程中理化特性和质地特性及挥发性化合物的影响。采用固相微萃取/气相色谱-质谱联用(SPME/GC-MS)法测定挥发性化合物。结果表明,5 种发酵剂生产的奶酪在成熟过程中水分含量降低,灰分含量增加。山羊奶酪的 pH 值在成熟过程中先降低后升高,其中 M2 奶酪的 pH 值最低。奶酪的硬度和咀嚼性随成熟度的增加而增加,而凝聚性、弹性和回复性则呈相反趋势。在 60d 时,Lp 发酵的奶酪咀嚼性、凝聚性、弹性和回复性最高,而 M2 发酵的奶酪硬度最高。通过 SPME/GC-MS 共鉴定出 53 种挥发性成分,羧酸、醇、酮和酯是奶酪特征风味的 4 种主要贡献者。不同奶酪中挥发性成分及其含量差异较大。M2 奶酪中主要挥发性化合物(90.10%)的相对含量最高,特别是丁酸和乙酰丙酮。通过综合比较结果,我们得出结论,M2 奶酪质地致密,奶香浓郁,M2 是山羊奶酪生产的潜在发酵剂候选物。