Defoliation-induced tree growth declines are jointly limited by carbon source and sink activities.

Defoliation resulting from herbivory, storm, drought, and frost may seriously impair tree growth and forest production. However, a comprehensive evaluation of defoliation impacts on tree carbon (C) assimilation and growth has not been conducted. We performed a meta-analysis of a dataset that included 1562 observations of 40 tree species from 50 studies worldwide, and evaluated defoliation impacts on photosynthetic capacity, C allocation, and tree growth. Our results showed that the reduced tree-level leaf area by defoliation outweighed the enhanced leaf-level photosynthesis, leading to a net reduction in tree C assimilation that was accompanied with decreases in nonstructural carbohydrates (NSCs) concentrations. The negative effects of defoliation on leaf NSCs decreased over time, but leaf production increased following defoliation, suggesting a shift in the C allocation towards shoots over roots. Defoliation intensity negatively affected tree growth, but post-defoliated recovery time did oppositely. The structure equation modelling showed that defoliation reduced tree growth mainly by indirectly reducing C assimilation (r = -0.4), and minorly by direct negative effect of defoliation intensity (r = -0.28) and positive effect of post-defoliated time (r = 0.33). These findings suggest that tree growth declines caused by defoliation are co-limited by C-source and sink activities, which provide a physiological basis of tree growth that is of significance in tree growth modelling and forest management under global changes.