Shimojima Yukako, Kodo Yukihiro, Soeda Kana, Koike Hiroshi, Kanda Maki, Hayashi Hiroshi, Nishino Yukari, Fukui Rie, Kuroda Sumiyo, Hirai Akihiko, Suzuki Jun, Sadamasu Kenji
Tokyo Metropolitan Institute of Public Health.
Sagami Women's University.
Shokuhin Eiseigaku Zasshi. 2020;61(5):178-182. doi: 10.3358/shokueishi.61.178.
To recognize the risk of Bacillus cereus in pasteurized milk, we investigated the prevalence of B. cereus and the rate of the production of cereulide from B. cereus isolates. B. cereus was found in 66 out of 101 (65.3%) domestically pasteurized milk samples in Japan. The ces gene was identified in 3 out of 90 B. cereus isolates that were isolated from three samples (one product) among the 101 samples. The ces gene positive isolate, the reference strain F4810/72 and a B. cereus isolate collected in a food poisoning incident were shown the productivity of cereulide using an LC-MS/MS analysis. The LC-MS/MS analysis was confirmed the ability of identification and quantification of cereulide produced in the milk samples. In this study, it was shown that B. cereus strains are prevalent in pasteurized milk, some of these strains produce cereulide, and confirmed usefulness of LC-MS/MS analysis to detect cereulide in milk.
为了认识巴氏杀菌乳中蜡样芽孢杆菌的风险，我们调查了蜡样芽孢杆菌的流行情况以及蜡样芽孢杆菌分离株产生蜡样毒素的比率。在日本国内101份巴氏杀菌乳样品中，有66份（65.3%）检测出蜡样芽孢杆菌。从101份样品中的3份样品（1个产品）分离出的90株蜡样芽孢杆菌中，有3株鉴定出了ces基因。通过液相色谱-串联质谱（LC-MS/MS）分析表明，ces基因阳性分离株、参考菌株F4810/72以及从一起食物中毒事件中分离出的一株蜡样芽孢杆菌均能产生蜡样毒素。LC-MS/MS分析证实了对乳样品中产生的蜡样毒素进行鉴定和定量的能力。在本研究中，结果表明蜡样芽孢杆菌菌株在巴氏杀菌乳中普遍存在，其中一些菌株能产生蜡样毒素，并且证实了LC-MS/MS分析在检测乳中蜡样毒素方面的实用性。
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