Li Jiarui, Chen Junxin, Hu Zhijun, Xu Wenbin
Department of Urology Surgery, The First Affiliated Hospital of Nanchang University, Medical College of Nanchang University, Nanchang, Jiangxi 330000, P.R. China.
Department of Orthopedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University & Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, Zhejiang 310016, P.R. China.
Oncol Lett. 2020 Dec;20(6):367. doi: 10.3892/ol.2020.12229. Epub 2020 Oct 15.
Osteosarcoma (OS) is a common bone tumor with high mortality worldwide. The long-term survival rate of patients with metastatic or recurrent disease is <20%. The present study explored the biological role of microRNA (miRNA/miR)-1236-3p in OS. miRNA and mRNA expression levels were measured via reverse transcription-quantitative PCR. Fluorescence hybridization was performed to determine miR-1236-3p expression levels in clinical specimens. Protein expression was measured via western blotting. Immunohistochemical analysis was used to detect Wnt target gene expression in tumor tissues. The interaction between the Wnt3a 3'untranslated region and miR-1236-3p was assessed via dual-luciferase reporter assays. Cell cycle, Transwell, Cell Counting Kit-8 and wound healing assays were conducted to evaluate the function of the miR-1236-3p/Wnt3a axis. Human OS (HOS) cells stably transfected with vector or miR-1236-3p sponge were injected subcutaneously into nude mice to assess the role of miR-1236-3p . miR-1236-3p expression was downregulated in OS tissues compared with chondroma tissues, and miR-1236-3p overexpression inhibited OS cell migration and proliferation compared with the negative control group. Furthermore, xenograft assays displayed enhanced tumour growth rates in the miR-1236-3p sponge group compared with the vector control group. In the present study, the results indicated that miR-1236-3p inhibited OS progression and Wnt3a was identified as a target of miR-1236-3p.
骨肉瘤（OS）是一种在全球范围内死亡率较高的常见骨肿瘤。转移性或复发性疾病患者的长期生存率<20%。本研究探讨了微小RNA（miRNA/miR）-1236-3p在骨肉瘤中的生物学作用。通过逆转录定量PCR检测miRNA和mRNA表达水平。进行荧光杂交以确定临床标本中miR-1236-3p的表达水平。通过蛋白质印迹法检测蛋白质表达。采用免疫组织化学分析检测肿瘤组织中Wnt靶基因的表达。通过双荧光素酶报告基因检测评估Wnt3a 3'非翻译区与miR-1236-3p之间的相互作用。进行细胞周期、Transwell、细胞计数试剂盒-8和伤口愈合实验以评估miR-1236-3p/Wnt3a轴的功能。将稳定转染载体或miR-1236-3p海绵的人骨肉瘤（HOS）细胞皮下注射到裸鼠体内，以评估miR-1236-3p的作用。与软骨瘤组织相比，骨肉瘤组织中miR-1236-3p表达下调，与阴性对照组相比，miR-1236-3p过表达抑制了骨肉瘤细胞的迁移和增殖。此外，异种移植实验显示，与载体对照组相比，miR-1236-3p海绵组的肿瘤生长速率加快。在本研究中，结果表明miR-1236-3p抑制骨肉瘤进展，并且Wnt3a被确定为miR-1236-3p的靶标。
