Department of Respiratory Medicine, Qilu Hospital of Shandong University, Qingdao, China.
Blood Transfusion Department, Qilu Hospital of Shandong University, Qingdao, China.
Biomed Res Int. 2020 Oct 20;2020:1975875. doi: 10.1155/2020/1975875. eCollection 2020.
() is an important opportunistic pathogen widely present in medical environment. Given its complex drug resistance, poses a serious threat to the safety of critically ill patients. Given the limited alternative antibiotics, nonantibiotic-based functional anti- infection proteins must be developed. In this study, we firstly used a series of biological software to predict potential epitopes in the MacB protein sequence and verified them by antibody recognition and lymphocyte proliferation tests. We finally screened out B cell epitope 2, CD8 T cell epitope 7, and CD4 T cell epitope 11 and connected them to construct a recombinant antigen epitope (RAE). The determination of IgG in the serum of immunised mice and cytokines in the supernatant of lymphocytes showed that the constructed epitope induced an immune response mediated by Th-1 cells. Finally, the challenge experiment of infection in mice confirmed that the epitope developed based on MacB, especially RAE, provided incomplete immune protection for mice.
（）是一种广泛存在于医疗环境中的重要机会性病原体。由于其复杂的耐药性，对重症患者的安全构成了严重威胁。鉴于抗生素的选择有限，必须开发非抗生素的功能性抗感染蛋白。在这项研究中，我们首先使用一系列生物软件预测 MacB 蛋白序列中的潜在表位，并通过抗体识别和淋巴细胞增殖试验进行验证。最后，我们筛选出 B 细胞表位 2、CD8 T 细胞表位 7 和 CD4 T 细胞表位 11，并将它们连接起来构建重组抗原表位（RAE）。免疫小鼠血清中的 IgG 和淋巴细胞上清液中的细胞因子的测定表明，构建的表位诱导了 Th-1 细胞介导的免疫反应。最后，对小鼠感染的攻毒实验证实，基于 MacB 开发的表位，特别是 RAE，为小鼠提供了不完全的免疫保护。
