Eadie Ashley, Vásquez Isabel Cristina, Liang Xuefang, Wang Xiaohong, Souders Christopher L, Chehouri Jana El, Hoskote Rohit, Feswick April, Cowie Andrew M, Loughery Jennifer R, Martyniuk Christopher J
Department of Biology, University of New Brunswick, Saint John, New Brunswick, E2L 4L5, Canada.
Departamento de Ciencias Fisiológicas, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá D.C., Colombia.
Data Brief. 2020 Oct 16;33:106413. doi: 10.1016/j.dib.2020.106413. eCollection 2020 Dec.
Fipronil is a phenylpyrazole pesticide that is used in both residential and agricultural applications. Fipronil is detected in run-off and water systems that are near areas in which the pesticide has been applied. The pesticide acts to antagonize gamma aminobutyric acid receptors, leading to over-excitation in the central nervous system. Fipronil has relatively high toxicity to fish, but the mechanisms underlying the toxicity are not well understood in embryonic stages. Zebrafish embryos were exposed to a single concentration of fipronil for 48 h at ∼3-4 h-post-fertilization. Following a 7-day depuration phase, transcriptome and behavioral analyses were conducted. Transcriptomics identified neural processes as those differentially expressed with different doses of fipronil (0.2 µg, 200 µg and 2 mg fipronil/L). Gene networks associated with astrocyte differentiation, myelination, neural tube development, brain stem response, innervation, nerve regeneration, astrocyte differentiation, among other pathways were altered with exposure. In addition, miRNA-related events are disrupted by fipronil exposure and genes associated with primary or pri-miRNA processing were increased in larval fish exposed to the pesticide. These data present putative mechanisms associated with neurological impacts at later ages of zebrafish. This is important because it is not clear how early exposure to pesticides like fipronil affect central nervous system function and organisms later in life.
氟虫腈是一种苯基吡唑类杀虫剂，用于住宅和农业领域。在施用该杀虫剂区域附近的径流和水系统中可检测到氟虫腈。该杀虫剂作用于拮抗γ-氨基丁酸受体，导致中枢神经系统过度兴奋。氟虫腈对鱼类具有较高毒性，但其在胚胎阶段的毒性作用机制尚不清楚。在受精后约3 - 4小时，将斑马鱼胚胎暴露于单一浓度的氟虫腈中48小时。经过7天的净化期后，进行转录组和行为分析。转录组学确定神经过程是在不同剂量的氟虫腈（0.2微克、200微克和2毫克氟虫腈/升）作用下差异表达的过程。与星形胶质细胞分化、髓鞘形成、神经管发育、脑干反应、神经支配、神经再生、星形胶质细胞分化等途径相关的基因网络因暴露而改变。此外，氟虫腈暴露会破坏与微小RNA相关的事件，并且在暴露于该杀虫剂的幼鱼中，与初级或原始微小RNA加工相关的基因增加。这些数据揭示了斑马鱼后期与神经学影响相关的假定机制。这很重要，因为目前尚不清楚早期接触氟虫腈等杀虫剂如何影响中枢神经系统功能以及生物体后期的生命活动。
