Mo Xuhua, Yang Song
Shandong Province Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
Curr Microbiol. 2021 Jan;78(1):107-113. doi: 10.1007/s00284-020-02272-0. Epub 2020 Nov 2.
The bacterium Saccharothrix syringae NRRL B-16468 is the producer of nocamycin I and nocamycin II which feature tetramic acid and bicyclic ketal groups. In this study, we presented the complete genome of S. syringae NRRL B-16468 obtained from ARS Culture Collection. It contains a circular chromosome of 10,929,570 bp with an average GC content of 73.49%, 9316 genes, 12 rRNAs and 54 tRNAs. Bioinformatics analyses of the genome has demonstrated that it harbors 55 putative biosynthetic gene clusters (BGCs) involved in synthesizing diverse secondary metabolites. The backbones of the natural products synthesized by these BGCs encoding for type I polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS) and hybrid type I PKS-NRPS were analyzed, furthermore, the natural products synthesized by these BGCs with > 40% similarity to known BGCs were described in detail. The complete genome of S. syringae reveals its capacity in producing diverse bioactive natural products, and it will also shed lights on mining novel secondary metabolites from S. syringae through rational strategies.
丁香糖丝菌NRRL B - 16468可产生诺卡霉素I和诺卡霉素II，其具有四氢吡咯酸和双环缩酮基团。在本研究中，我们展示了从美国农业研究局菌种保藏中心获得的丁香糖丝菌NRRL B - 16468的完整基因组。它包含一条10,929,570 bp的环状染色体，平均GC含量为73.49%，有9316个基因、12个rRNA和54个tRNA。对该基因组的生物信息学分析表明，它含有55个推定的生物合成基因簇（BGC），参与合成多种次级代谢产物。分析了这些编码I型聚酮合酶（PKS）、非核糖体肽合成酶（NRPS）和I型PKS - NRPS杂合体的BGC所合成的天然产物的骨架，此外，还详细描述了这些与已知BGC相似度大于40%的BGC所合成的天然产物。丁香糖丝菌的完整基因组揭示了其产生多种生物活性天然产物的能力，也将为通过合理策略从丁香糖丝菌中挖掘新型次级代谢产物提供线索。
