Department of Cardiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
J Cardiovasc Pharmacol. 2021 Jan 1;77(1):94-99. doi: 10.1097/FJC.0000000000000926.
Embryonic epicardial cells make an important contribution to cardiac development. However, their proliferation mechanism is still unclear. Epicardial cells from E12.5 fetal hearts were used in our study. Agrin was used to treat these cells. The expression of Aurora B, Ki67, and pH3 was measured by quantitative reverse transcription-polymerase chain reaction and immunofluorescence. The proportion of cells in G1/S/G2 phase was determined by flow cytometry. The results showed that agrin significantly increased the expression of ki67, pH3, and Aurora B in epicardial cells. Flow cytometry results showed that agrin significantly increased the proportion of epicardial cells in S phase. However, blocking yes-associated protein significantly downregulated the levels of ki67, pH3, and Aurora B and the proportion of epicardial cells in S phase. Thus, our results suggest that agrin may promote the proliferation of epicardial cells by regulating the yes-associated protein activity. This may be useful in exploring heart development mechanisms and preventing congenital heart disease.
胚胎心外膜细胞对心脏发育有重要贡献。然而，其增殖机制尚不清楚。本研究使用 E12.5 胎鼠心外膜细胞，用 Agrin 处理这些细胞。通过定量逆转录聚合酶链反应和免疫荧光法测量 Aurora B、Ki67 和 pH3 的表达。通过流式细胞术测定 G1/S/G2 期细胞的比例。结果表明 Agrin 可显著增加心外膜细胞中 Ki67、pH3 和 Aurora B 的表达。流式细胞术结果表明 Agrin 可显著增加心外膜细胞在 S 期的比例。然而，阻断 Yes 相关蛋白可显著下调 Ki67、pH3 和 Aurora B 的水平以及心外膜细胞在 S 期的比例。因此，我们的结果表明 Agrin 可能通过调节 Yes 相关蛋白的活性来促进心外膜细胞的增殖。这对于探索心脏发育机制和预防先天性心脏病可能是有用的。
