College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, Henan, China.
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, Henan, China.
Microb Pathog. 2021 Mar;152:104601. doi: 10.1016/j.micpath.2020.104601. Epub 2020 Nov 1.
Interferon regulatory factors (IRFs) are a family of transcription factors that play a role in a variety of biological processes including immune regulation of interferon and expression of inflammatory cytokines. However, the data on IRFs are rather limited in chickens. In the present study, qRT-PCR was used to study the tissue distribution of IRFs in chickens at D15 (the 15th day of raising) and developmental changes of all chIRFs (Chicken interferon regulatory factors) in BF from E15 (the 15th day of incubation) to D15. The effects of IBDV infection with chickens on the transcriptional level of chIRFs were also investigated. The results showed: (1) chIRF1 mRNA was expressed much more abundantly in intestinal tract, chIRF2, chIRF6, chIRF7, chIRF8 and chIRF10 distributed mainly in liver or/and kidney. The expression of chIRF5 was mainly in spleen and chIRF4 distributed uniquely abundantly in BF. (2) The mRNA expression levels of chIRF5, chIRF7, chIRF8 and chIRF10 was low before hatching of chicken and at D1 and increased significantly from D5 till to the experiment end and the fold change of chIRF5 at D10 and chIRF7 at D5 reached 41.0-fold and 15.7-fold compared to that of E15, respectively (P < 0.05). ChIRF4 mRNA level was always high during the whole experiment except for E15 and it was 11.9-fold at the highest time point than that of E15 (the lowest time point). (3) When chicken was infected with IBDV, the expression levels of chIRF2, chIRF7 and chIRF10 mRNA had the tendency of increasing first and then decreasing but they peaked at 1dpi, 2 dpi, and 3dpi, respectively. The expression of chIRF5 mRNA was suppressed obviously during the whole experiment stage in IBDV-infected chicken. And chIRF4 expression was up-regulated transitorily at 1dpi and then was suppressed on a very low level till to the experiment end. Conclusion: The chIRFs were constitutively expressed in different tissues examined and has tissue-specific expression. Of them, chIRF2, chIRF4, chIRF5, chIRF7, chIRF8 and chIRF10 were related closely with the development or immune response of BF, and when chicken was infected with IBDV, some of them were activated, earlier or later on, some of them were suppressed. These findings would help to sieve out a few antiviral chIRF candidate gene to improve the host's innate immune and provide a foundation of the further exploiting a new vaccine adjuvant.
干扰素调节因子（IRFs）是一类转录因子，在多种生物学过程中发挥作用，包括干扰素的免疫调节和炎症细胞因子的表达。然而，鸡的 IRF 数据相当有限。在本研究中，采用 qRT-PCR 技术研究了 D15 日龄（饲养第 15 天）鸡组织中 IRFs 的分布以及从 E15 日龄（孵化第 15 天）到 D15 日龄鸡 BF 中所有 chIRFs（鸡干扰素调节因子）的发育变化。还研究了 IBDV 感染对 chIRFs 转录水平的影响。结果表明：（1）chIRF1 mRNA 在肠道中表达丰度较高，chIRF2、chIRF6、chIRF7、chIRF8 和 chIRF10 主要分布在肝脏或/和肾脏中。chIRF5 的表达主要在脾脏中，chIRF4 则在 BF 中表达丰度独特。（2）chIRF5、chIRF7、chIRF8 和 chIRF10 的 mRNA 表达水平在鸡孵化前和 D1 时较低，从 D5 到实验结束时显著增加，chIRF5 在 D10 和 chIRF7 在 D5 的表达变化倍数分别达到 41.0 倍和 15.7 倍（P < 0.05）。chIRF4 的 mRNA 水平在整个实验过程中一直较高，除了 E15 之外，在最高时间点比 E15 高 11.9 倍（最低时间点）。（3）当鸡感染 IBDV 时，chIRF2、chIRF7 和 chIRF10 的 mRNA 表达水平呈先增加后减少的趋势，但均在 1dpi、2dpi 和 3dpi 时达到峰值。在整个 IBDV 感染鸡的实验阶段，chIRF5 的 mRNA 表达受到明显抑制。chIRF4 的表达在 1dpi 时短暂上调，然后一直被抑制在非常低的水平直到实验结束。结论：chIRFs 在不同检测组织中持续表达，具有组织特异性表达。其中，chIRF2、chIRF4、chIRF5、chIRF7、chIRF8 和 chIRF10 与 BF 的发育或免疫反应密切相关，当鸡感染 IBDV 时，它们中的一些被激活，早或晚，其中一些被抑制。这些发现有助于筛选出一些抗病毒的 chIRF 候选基因，以提高宿主的固有免疫，并为进一步开发新的疫苗佐剂提供基础。
