Alvi Mughees Aizaz, Ohiolei John Asekhaen, Li Li, Saqib Muhammad, Hussain Muhammad Hammad, Tayyab Muhammad Haleem, Ghafoor Muzafar, Qamar Warda, Faridi Noman Yousaf, Alvi Anum Aizaz, Fu Bao-Quan, Yan Hong-Bin, Jia Wan-Zhong
State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad 38400, Pakistan.
Pathogens. 2020 Oct 29;9(11):905. doi: 10.3390/pathogens9110905.
Cystic echinococcosis (CE) is a World Health Organization (WHO)-listed neglected tropical farm economy jeopardizing and public health concern disease. This study was aimed at furnishing sero-epidemiological baseline data of CE in sheep in Pakistan, where data are non-existent. For this purpose, two sheep-rich provinces of Pakistan were selected, and 728 sheep sera were collected using probability proportional to size (PPS) statistical technique. Epidemiological information was recorded on a questionnaire for the estimation of potential risk factors. The serum samples were analyzed for IgG antibodies against using an in-house-developed EgAgB-based ELISA kit. The overall seroprevalence recorded was 21.98% (160/728) in the tested sheep, suggesting higher seropositivity in sheep from Punjab (23.73%) as compared to Khyber Pakhtunkhwa (KPK) (19.04%). The overall apparent prevalence observed by this ELISA method was almost similar to the calculated true prevalence (21.77%). Prevalence was significantly different ( < 0.05) among sheep from different districts. Higher prevalence was found in females (22.54%, OR 1.41), age group > 5 years (29.66%, OR 1.64), crossbreeds (42.85%, OR 2.70), and sheep with pasture access (25.96%, OR 3.06). Being in age group > 5 years and having pasture access were the factors significantly associated with seropositivity ( < 0.05). This study provides serological evidence of . infection in sheep and can be used as a model for screening of the sheep globally.
囊型包虫病（CE）是世界卫生组织（WHO）列为被忽视的热带病，对农业经济和公共卫生构成危害。本研究旨在提供巴基斯坦绵羊中囊型包虫病的血清流行病学基线数据，该国尚无相关数据。为此，选择了巴基斯坦绵羊数量较多的两个省份，采用按规模大小概率抽样（PPS）统计技术收集了728份绵羊血清。通过问卷调查记录流行病学信息，以评估潜在风险因素。使用自行研发的基于细粒棘球绦虫抗原B（EgAgB）的酶联免疫吸附测定（ELISA）试剂盒分析血清样本中的IgG抗体。在所检测的绵羊中，总体血清阳性率为21.98%（160/728），表明旁遮普省绵羊的血清阳性率（23.73%）高于开伯尔-普赫图赫瓦省（KPK）（19.04%）。通过该ELISA方法观察到的总体表观患病率与计算出的真实患病率（21.77%）几乎相似。不同地区绵羊的患病率存在显著差异（P<0.05）。雌性绵羊（22.54%，比值比1.41）、年龄大于5岁的年龄组（29.66%，比值比1.64）、杂交品种（42.85%，比值比2.70）以及可进入牧场的绵羊（25.96%，比值比3.06）的患病率较高。年龄大于5岁且可进入牧场是与血清阳性显著相关的因素（P<0.05）。本研究提供了绵羊感染细粒棘球绦虫的血清学证据，可作为全球绵羊细粒棘球绦虫筛查的模型。
