Department of Pathology, ISGlobal, Hospital Clínic-Universitat de Barcelona, 08036 Barcelona, Spain.
Unit of Infections and Cancer, Cancer Epidemiology Research Program, Catalan Institute of Oncology, IDIBELL, 08908 L'Hospitalet de Llobregat, Spain.
Int J Mol Sci. 2020 Oct 29;21(21):8091. doi: 10.3390/ijms21218091.
Human papillomavirus (HPV)-independent vulvar squamous cell carcinomas (VSCC) and its precursors frequently harbour mutations. Recently, six p53 immunohistochemical (IHC) patterns have been defined, which have shown strong correlation with mutation status. However, few studies have applied this new six-pattern framework and none of them exhaustively compared p53 IHC positivity and patterns between invasive VSCC and adjacent skin lesion. We performed p53 IHC in a series of 779 HPV-independent VSCC with adjacent skin and evaluated the IHC slides following the newly described classification. Some 74.1% invasive VSCC showed abnormal p53 IHC staining. A skin lesion was identified in 450 cases (57.8%), including 254 intraepithelial precursors and 196 inflammatory/reactive lesions. Two hundred and ten of 450 (47%) VSCC with associated skin lesions showed an abnormal p53 IHC stain, with an identical staining pattern between the VSCC and the adjacent skin lesion in 80% of the cases. A total of 144/450 (32%) VSCC showed wild-type p53 IHC both in the invasive VSCC and adjacent skin lesion. Finally, 96/450 (21%) VSCC showed p53 IHC abnormal staining in the invasive VSCC but a wild-type p53 staining in the skin lesion. Most of the discordant cases (70/96; 73%) showed adjacent inflammatory lesions. In conclusion, the p53 IHC staining and pattern are usually identical in the VSCC and the intraepithelial precursor.
人乳头瘤病毒（HPV）非依赖性外阴鳞状细胞癌（VSCC）及其前体通常存在 突变。最近，已经定义了六种 p53 免疫组织化学（IHC）模式，这些模式与 突变状态具有很强的相关性。然而，很少有研究应用这种新的六模式框架，也没有研究穷尽地比较浸润性 VSCC 与相邻皮肤病变之间的 p53 IHC 阳性率和模式。我们对 779 例 HPV 非依赖性 VSCC 及其相邻皮肤进行了 p53 IHC 检测，并按照新描述的分类对 IHC 切片进行了评估。一些 74.1%的浸润性 VSCC 显示出异常的 p53 IHC 染色。在 450 例病例中发现了皮肤病变（57.8%），包括 254 例上皮内前体和 196 例炎症/反应性病变。在 450 例 VSCC 中，有 210 例（47%）伴有皮肤病变显示异常的 p53 IHC 染色，其中 80%的病例 VSCC 与相邻皮肤病变具有相同的染色模式。在 450 例 VSCC 中，共有 144 例（32%）显示出侵袭性 VSCC 和相邻皮肤病变中 p53 IHC 野生型。最后，96 例（21%）VSCC 在侵袭性 VSCC 中显示 p53 IHC 异常染色，但在皮肤病变中显示 p53 IHC 野生型。大多数不一致的病例（70/96；73%）显示为相邻的炎症性病变。总之，VSCC 与上皮内前体中的 p53 IHC 染色和模式通常是一致的。
